文献类型：期刊文献

英文题名：Rapid electrochemical detection of domoic acid based on polydopamine/reduced graphene oxide coupled with in-situ imprinted polyacrylamide

作者：Jiang, Mengnan[1];Tang, Junyan[1];Zhou, Nuoyi[1];Liu, Juan[1];Tao, Feifei[3];Wang, Fei[1];Li, Caolong[1,2]

机构：[1]China Pharmaceut Univ, Sch Sci, Key Lab Biomed Funct Mat, Nanjing 211198, Peoples R China;[2]Tibetan Tradit Med Coll, Tibetan Med Res Inst, Lhasa 850000, Tibet, Peoples R China;[3]Shaoxing Univ, Dept Chem & Chem Engn, Shaoxing 312000, Peoples R China

年份：2022

卷号：236

外文期刊名：TALANTA

收录：SCI-EXPANDED(收录号：WOS:000697556900004)、、EI(收录号：20213810906616)、Scopus(收录号：2-s2.0-85114914354)、WOS

基金：This work was supported by the National Key Research and Development Program (2019YFC0312602), the National Innovation and Entrepreneurship Training Program for Undergraduate (201910316205) and the Natural Science Foundation of China (51372154).

语种：英文

外文关键词：Molecularly imprinted polymer; Electrochemical sensor; Domoic acid; Graphene; Polydopamine; Screen-printed electrode

外文摘要：Domoic acid, namely amnesic shellfish toxin, is a highly neurotoxic substance to marine animals and humankind. To reduce the incidence of poisoning accidents, the exploitation of specific and rapid detection method for domoic acid monitoring is highly required. Herein, an electrochemical molecularly imprinted polymer (MIP) sensor based on polydopamine-reduced graphene oxide/polyacrylamide composite (PDA-rGO/PAM) was constructed successfully to detect domoic acid. The domoic acid molecule could be recognized in imprinted cavities of PAM reversibly through hydrogen bonding. PDA-rGO promoted the loading capacity of PAM and improved the charge transfer rate, which amplified the electrical signal response of the MIP sensor. The screen-printed electrode (SPE) modified with PDA-rGO/PAM displayed satisfactory response toward toxin contaminated sample at a linear range from 1 to 600 nM and a low detection limit of 0.31 nM, demonstrating the prospective application of the transducer as a portable sensing platform for the on-site detection of hazardous marine biotoxin. Moreover, benefiting from the superior specificity and stability of MIP, the fabricated sensor could be utilized to detect the domoic acid content in mussel extracts directly without complex pretreatment operation.