文献类型：期刊文献

中文题名：nNOS、VEGF和Bcl-2蛋白在人胚胎发育中脊髓前角的表达及意义

英文题名：Expression and significance of nNOS,VEGF and Bcl-2 proteins in the developing anterior horn of human embryonic spinal cord

作者：刘学红[1];张泳[1];夏国园[1];俞爱月[1]

机构：[1]绍兴文理学院医学院

年份：2009

期号：3

起止页码：496

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD、、Scopus、CSCD2011_2012、北大核心2008、北大核心、CSCD

语种：中文

中文关键词：脊髓;神经元型一氧化氮合酶;B细胞淋巴瘤/白血病-2;血管内皮生长因子;免疫组织化学;人胚胎

外文关键词：Spinal cord; Neuronal nitric oxide synthase; B-cell lymphoma/leukemia-2; Vascular endothelial growth factor; Immunohistochemistry ; Human embryo

中文摘要：目的探讨神经元型一氧化氮合酶（nNOS）、血管内皮生长因子（VEGF）和B细胞淋巴瘤/白血病-2（Bcl-2）蛋白在人胚胎发育早期脊髓前角中的分布规律及其表达意义。方法应用免疫组织化学法检测第2、3、4月龄段,人胚胎脊髓前角中nNOS、VEGF和Bcl-2蛋白的表达。结果在第2-4个月龄段,nNOS和Bcl-2蛋白在人胚胎脊髓前角组织细胞中由弱阳性表达逐渐变为阳性表达。在第2个月龄段,VEGF蛋白在人胚胎脊髓前角组织中均呈阴性表达;第3-4个月龄段,VEGF蛋白在人胚胎脊髓前角组织细胞中由弱阳性表达逐渐变为阳性表达。结论nNOS、Bcl-2和VEGF蛋白与人胚胎脊髓前角神经元的生长发育关系密切。

外文摘要：Objective To explore the rule of distribution and expression significance of neuronal nitric oxide synthase （nNOS）, vascular endothelial growth factor （VEGF） and B-cell lymphoma/leukemia-2（Bcl-2）proteins in the developing spinal cord anterior horn of human embryos. Methods In the fourth month of gestation, the expression productions of nNOS, VEGF and Bel-2 proteins were investigated in the anterior horn of human embryonic spinal cord using the immunohistochemical methods. Results The intensities of the immunohistochemical signals of both nNOS and Bcl-2 proteins were progressively enhanced from the second to the fourth month of gestation. There was negative immunohistochemical signals of VEGF in the anterior horn of human embryonic spinal cord in the second month of gestation. At the third to fourth month of gestation, the expression of VEGF protein was from weak to strong in the anterior horn of human embryonic spinal cord. Conclusion nNOS, VEGF and Bcl-2 proteins are related to the growth and development of neurons in the spinal cord anterior horn of human embryos.