文献类型：期刊文献

英文题名：miR-202 functions as a tumor suppressor in hepatocellular carcinoma by targeting HK2

作者：Wang, Jiangang[1];Chen, Jili[1];Sun, Fang[1];Wang, Zhiwei[1];Xu, Wenfang[1];Yu, Yafeng[1];Ding, Feng[1];Shen, Huajiang[1]

机构：[1]Shaoxing Univ, Dept Liver Dis, Affiliated Hosp, 999 Zhongxing South Rd, Shaoxing 312000, Zhejiang, Peoples R China

年份：2020

卷号：19

期号：3

起止页码：2265

外文期刊名：ONCOLOGY LETTERS

收录：SCI-EXPANDED(收录号：WOS:000519933800070)、、Scopus(收录号：2-s2.0-85078767097)、WOS

语种：英文

外文关键词：hepatocellular carcinoma; microRNA-202; hexokinase 2; cell proliferation; glycolysis

外文摘要：Recent evidence has suggested that microRNAs (miRNAs) can participate in metabolic reprogramming. Additionally, aerobic glycolysis is associated with tumor progression in hepatocellular carcinoma (HCC). In the present study, miRNA (miR)-202 expression levels were found to be significantly lower in HCC tissues compared with the corresponding adjacent non-cancerous tissue samples using reverse transcription-quantitative PCR analysis in 56 patients with HCC. Lower miR-202 expression levels were identified to be associated with tumor size, vascular invasion, Tumor, Node and Metastasis stages and poor overall survival rates in patients with HCC. In vitro, upregulation of miR-202 expression was revealed to significantly suppress the cell glucose uptake, lactate production and cell proliferation in liver cancer cells. In addition, dual luciferase reporter analysis and western blot assays suggested that hexokinase 2 (HK2) was a direct target of miR-202. Upregulation of miR-202 expression could inhibit cell proliferation by regulating HK2 expression in HCC. Therefore, the results from the present study suggested that miR-202 may serve as a potential target for HCC treatment.