文献类型：期刊文献

英文题名：Participation of FaTRAB1 Transcription Factor in the Regulation of FaMADS1 Involved in ABA-Dependent Ripening of Strawberry Fruit

作者：Lu, Wenjing[1,2];Wei, Xiaopeng[2,3];Han, Xueyuan[2,4];Chen, Renchi[2];Xiao, Chaogeng[1];Zheng, Xiaojie[5];Mao, Linchun[2,6]

机构：[1]Zhejiang Acad Agr Sci, Inst Food Sci, 298 Desheng Rd, Hangzhou 310021, Peoples R China;[2]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Zhejiang Key Lab AgroFood Proc, Hangzhou 310058, Peoples R China;[3]Zhengzhou Univ Light Ind, Sch Food & Bioengn, Zhengzhou 450002, Peoples R China;[4]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Peoples R China;[5]Wenzhou Vocat Coll Sci & Technol, Dept Agr & Biotechnol, Wenzhou 325006, Peoples R China;[6]Zhejiang Univ, Ningbo Res Inst, Ningbo 315100, Peoples R China

年份：2023

卷号：12

期号：9

外文期刊名：FOODS

收录：SCI-EXPANDED(收录号：WOS:000986513300001)、、WOS

基金：This research was funded by the National Natural Science Foundation of China (31972468).

语种：英文

外文关键词：phytohormone; Fragaria x ananassa; fruit ripening; MADS; TRAB

外文摘要：Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of FaMADS1. The transient silence of FaMADS1 in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related PAL6, C4H, 4CL, DFR, and UFGT, softening-related PL and XTH, and aroma-related QR and AAT2. In addition, the interaction between FaMADS1 and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the FaMADS1 promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the FaMADS1 promoter, resulting in the decreased expression of FaMADS1. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting FaMADS1 expression by the active binding of transcript FaTRAB1 with the FaMADS1 promoter.