文献类型：期刊文献

英文题名：Panax Notoginseng Saponins Protect H9c2 Cells From Hypoxia-reoxygenation Injury Through the Forkhead Box O3a Hypoxia-inducible Factor-1 Alpha Cell Signaling Pathway

作者：Liu, Xin-Wen[1];Lu, Meng-Kai[1];Zhong, Hui-Ting[2];Liu, Jing-Jing[3];Fu, Yong-Ping[3]

机构：[1]Shaoxing Univ, Dept Pharm, Affiliated Hosp, Shaoxing, Peoples R China;[2]Shaoxing Univ, Dept Res, Affiliated Hosp, Shaoxing, Peoples R China;[3]Shaoxing Univ, Dept Cardiovasc Med, Affiliated Hosp, 999 Zhongxing South Rd, Shaoxing 312000, Peoples R China

年份：2021

卷号：78

期号：5

起止页码：e681

外文期刊名：JOURNAL OF CARDIOVASCULAR PHARMACOLOGY

收录：SCI-EXPANDED(收录号：WOS:000714687500008)、、WOS

基金：The authors gratefully acknowledge the financial support provided by the Natural Science Foundation of Zhejiang Province (No. LY18H020008), Medical Science and Technology Foundation of Zhejiang Province (No. 2019RC297), and Shaoxing Science and Technology Project (No. 2018C30029).

语种：英文

外文关键词：PNS; HIF-l alpha; FOXO3 alpha; autophagy; apoptosis

外文摘要：Panax notoginseng saponins (PNS) are commonly used in the treatment of cardiovascular diseases. Whether PNS can protect myocardial ischemia-reperfusion injury by regulating the forkhead box O3a hypoxia-inducible factor-1 alpha (FOXO3 alpha/HIF-1 alpha) cell signaling pathway remains unclear. The purpose of this study was to investigate the protective effect of PNS on H9c2 cardiomyocytes through the FOXO3 alpha/HIF-1 alpha cell signaling pathway. Hypoxia and reoxygenation of H9C2 cells were used to mimic MIRI in vitro, and the cells were treated with PNS, 2-methoxyestradiol (2ME2), and LY294002." Cell proliferation, lactate dehydrogenase, and malonal-dehyde were used to evaluate the degree of cell injury. The level of reactive oxygen species was detected with a fluorescence microscope. The apoptosis rate was detected by flow cytometry. The expression of autophagy-related proteins and apoptosis-related proteins was detected by western blot assay. PNS could reduce H9c2 hypoxia-reoxygenation injury by promoting autophagy and inhibiting apoptosis through the HIF-1 alpha/FOXO3 alpha cell signaling pathway. Furthermore, the protective effects of PNS were abolished by HIF-1 alpha inhibitor 2ME2 and PI3K/Akt inhibitor LY294002. PNS could reduce H9c2 hypoxia-reoxygenation injury by promoting autophagy and inhibiting apoptosis through the HIF-1 alpha/FOXO3 alpha cell signaling pathway.