文献类型：期刊文献

英文题名：Wogonoside alleviates the proliferation and promotes the apoptosis in liver cancer cells by regulating PI3K/Akt signaling pathway

作者：Li, Xinfang[1,2];Huang, Yitong[3];He, Yibo[4];Ye, Angzhi[5,6]

机构：[1]Wenzhou Med Univ, Zhuji Affiliated Hosp, Dept Clin Lab, Shaoxing 311800, Zhejiang, Peoples R China;[2]Shaoxing Univ, Zhuji Peoples Hosp Zhejiang Prov, Shaoxing 311899, Zhejiang, Peoples R China;[3]Zhuji Maternal & Child Hlth Hosp, Dept Internal Med, Shaoxing 311899, Zhejiang, Peoples R China;[4]Zhejiang Chinese Med Univ, Affiliated Hosp 1, Zhejiang Prov Hosp Chinese Med, Dept Clin Lab, Hangzhou 310003, Zhejiang, Peoples R China;[5]Zhuji Cent Hosp, Dept Clin Lab, Zhugong Rd 98, Shaoxing 311800, Zhejiang, Peoples R China;[6]Zhejiang Chinese Med Univ, Hangzhou 310053, Zhejiang, Peoples R China

年份：2025

卷号：16

期号：1

外文期刊名：DISCOVER ONCOLOGY

收录：SCI-EXPANDED(收录号：WOS:001434993500003)、、Scopus(收录号：2-s2.0-85218750985)、WOS

基金：Not applicable.

语种：英文

外文关键词：Wogonoside; Liver cancer; PI3K/Akt signaling pathway; Proliferation; Apoptosis

外文摘要：IntroductionPrimary liver cancer is associated with high morbidity and mortality rate. In about 50% of cases, primary liver cancer is related to the phosphoinositide-3-kinase (PI3K)/Akt signaling pathway. Wogonoside is an active component extracted from Scutellaria baicalensis. Its antitumor effects in liver cancer are scarcely known.AimThis study explores the correlation between wogonoside and the PI3K/Akt signaling pathway in liver cancer in vitro.MethodsTHLE-2 cells and HepG2 cells were treated with different concentrations of wogonoside to establish low-, medium- and high- dose groups, and the concentration of each dose group was determined by CCK-8 assay. Subsequent experiments were evaluated the viability, proliferation, invasion, wound healing, apoptosis rate of HepG2 cells, as well as the expression levels of relevant targets. In silico network pharmacology was performed to investigate the relationship between wogonoside and the PI3K/Akt signaling pathway, providing insights into the connection between wogonoside and liver cancer.ResultsCompared with the control group, the viability, proliferation, invasion, migration and wound healing ability of wogonoside-treated HepG2 cells were significantly declined in a dose- and time-dependent manner. Wogonoside significantly reduced the relative expression level of Bcl-2/Bax relative protein. Wogonoside also decreased the relative protein expression of phospho-PI3K/PI3K and phospho-AKT/AKT and the mRNA levels of PI3K and AKT. In addition, potential key genes, biological processes, and pathways associated with the therapeutic effects of wogonoside on liver cancer were explored.ConclusionWogonoside can alleviate the proliferation and promote the apoptosis of HepG2 cells, which may be related to the PI3K/Akt signaling pathway.