文献类型：期刊文献

英文题名：Profiling of exosomal microRNAs expression in umbilical cord blood from normal and preeclampsia patients

作者：Pan, Hai-Tao[1,2,3];Shi, Xiao-Liang[1,2];Fang, Min[1,2];Sun, Xiang-Mei[2];Chen, Pan-Pan[2];Ding, Jin-Long[1,2];Xia, Gui-Yu[1,2];Yu, Bin[1,2];Zhang, Tao[1,2];Zhu, Hong-Dan[1,2]

机构：[1]Shaoxing Matern & Child Hlth Care Hosp, Shaoxing 312000, Peoples R China;[2]Shaoxing Univ, Obstet & Gynecol Hosp, Shaoxing, Peoples R China;[3]Shanghai Jiao Tong Univ, Int Peace Matern & Child Hlth Hosp, Sch Med, Shanghai 200030, Peoples R China

年份：2022

卷号：22

期号：1

外文期刊名：BMC PREGNANCY AND CHILDBIRTH

收录：SCI-EXPANDED(收录号：WOS:000754666600002)、、Scopus(收录号：2-s2.0-85124583992)、WOS

基金：This work was supported by the National Natural Science Foundation of China (82071729), the China Postdoctoral Science Foundation (2020 M681336), the Science Technology Department of Zhejiang Province, China (LGF21H040004, LGF19H040004, LGD20H040001) and the Health Commission of Zhejiang Province, China (2022RC277, 2022KY413, 2021KY375, 2019RC296, 2019KY229, 2019KY717); the Science Technology Department of Shaoxing, China (2020A13034, 2020A13035, 2018C30050).

语种：英文

外文关键词：Preeclampsia; Umbilical serum; Exosomal miRNAs; Offspring; Microvascular dysfunction; Gamete and embryo-fetal origins of adult diseases

外文摘要：Background Epidemiological and experimental studies suggest that preeclampsia has a negative impact on maternity and offspring health. Previous studies report that dysregulation in utero-environment increases risk for elderly disease such as cardiovascular disease. However, the underlying mechanisms remain elusive. Specific microRNAs (miRNAs) are packaged in exosomes may regulate microvascular dysfunction in offspring of mothers with preeclampsia. The present study aimed to identify the differential expression profiles of microRNAs in the serum exosomes between patients with preeclampsia and normal pregnancies. Methods A comprehensive miRNA sequence-based approach was performed to compare exosomes carry miRNAs (Exo-miRNAs) expression levels in umbilical serum between normal and preeclampsia patients. Exosomes were isolated using the ExoQuick precipitation kit. Serum exosomes were then viewed under electron microscopy, and their characteristics determined by western blotting and nanoparticle-tracking analysis. Illumina platform was used to perform sequencing. Bioinformatics analysis was used to explore differentially expressed Exo-miRNAs in umbilical serum. Results Based on sequence similarity, 1733 known miRNAs were retrieved. Furthermore, 157 mature miRNAs in serum exosomes were significantly differential expressed between PE and those control groups (P<0.05, log(2)|FC| > 1). Out, of the 157 miRNAs, 96 were upregulated miRNAs whereas 61 miRNAs were downregulated. The 157 differentially expressed miRNAs targeted 51,424 differentially expressed genes. Functional analysis through KEGG pathway and Gene Ontology results uncovered that target genes of miRNAs with differential expression were significantly linked to several pathways and biological processes. Conclusion The findings of this study showed differential expression of umbilical serum Exo-miRNAs in normal compared with PE patients, implying that these Exo-miRNAs may associate with microvascular dysfunction in offspring of mothers with preeclampsia.