文献类型：期刊文献

英文题名：Correlation analysis of peripheral DPYD gene polymorphism with 5-fluorouracil susceptibility and side effects in colon cancer patients

作者：Sun, Wenhui[1];Yan, Chungen[2];Jia, Shaochang[3,4];Hu, Jianhua[3,4]

机构：[1]Fuyang Peoples Hosp, Dept Surg Oncol, Fuyang, Peoples R China;[2]Shaoxing Univ, Dept Gastroenterol, Affiliated Hosp, Shaoxing 312000, Zhejiang, Peoples R China;[3]PLA, Hosp 81, Dept Biotherapy, Nanjing 210002, Jiangsu, Peoples R China;[4]Jingdu Hosp, Nanjing 210002, Jiangsu, Peoples R China

年份：2014

卷号：7

期号：12

起止页码：5857

外文期刊名：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE

收录：SCI-EXPANDED(收录号：WOS:000348303600164)、、Scopus(收录号：2-s2.0-84921458906)、WOS

语种：英文

外文关键词：Colon cancer; dihydropyrimidine dehydrogenase; gene polymorphism; 5-fluorouracil

外文摘要：Objective: To investigate the correlation of peripheral DPYD gene polymorphism with 5-fluorouracil (5-FU) susceptibility and side effect in patients with colon cancer. Methods: The total DNA of peripheral mononuclear cells was extracted in 100 cases of colon cancer patients. Quantitative PCR was conducted to measure DPYD gene 14G1A, A1627G, T85C 3 loci polymorphism, and analyze the correlation of 5-FU susceptibility, side effects with DPYD gene polymorphism. Results: Mutations were detected at position 14G1A (mutation rate 14%), A1627G (mutation rate 11%), and T85C (mutation rate 17%). The effective rate of 3 loci of wild type was significantly higher than that of mutant type (P < 0.05). With respect to side effects such as myelosuppression, hand-foot syndrome, diarrhea, and gastrointestinal reactions, the incidence in mutation type was significantly higher than that in the wild type (P < 0.05). Conclusion: DPYD gene polymorphism plays a guiding role in predicting efficacy and toxicity of 5-FU, which can be used as an important reference index of 5-FU individualized administration scheme.