文献类型：期刊文献

英文题名：Increasing pesticide diversity impairs soil microbial functions

作者：Ni, Bang[1,2];Xiao, Lu[3];Lin, Da[1,2,4];Zhang, Tian- Lun[1,2,4];Zhang, Qi[5];Liu, Yanjie[3];Chen, Quan[6];Zhu, Dong[1,2];Qian, Haifeng[7];Rillig, Matthias C.[8,9];Zhu, Yong- Guan[1,2,4,10]

机构：[1]Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Ningbo Urban Environm Observat & Res Stn, Xiamen 361021, Peoples R China;[2]Chinese Acad Sci, Zhejiang Key Lab Urban Environm Proc & Pollut Cont, Haixi Ind Technol Innovat Ctr Beilun, Ningbo 315830, Peoples R China;[3]Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Wetland Ecol & Environm, State Key Lab Black Soils Conservat & Utilizat, Changchun 130102, Peoples R China;[4]Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China;[5]Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China;[6]Kunming Univ Sci &Technol, Fac Environm Sci & Engn, Yunnan Prov Key Lab Soil Carbon Sequestrat & Pollu, Kunming 650500, Peoples R China;[7]Zhejiang Univ Technol, Coll Environm, Hangzhou 310032, Peoples R China;[8]Free Univ Berlin, Inst Biol, D-14195 Berlin, Germany;[9]Berlin Brandenburg Inst Adv Biodivers Res, D-14195 Berlin, Germany;[10]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China

年份：2025

卷号：122

期号：2

外文期刊名：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

收录：SCI-EXPANDED(收录号：WOS:001416111500008)、、Scopus(收录号：2-s2.0-85214911235)、WOS

基金：Our work was supported by the National Natural Science Foundation of China (41991332, 42307027, and 42222701) , Ningbo Yongjiang Talent Project (2022A-163-G) , Ningbo Science and Technology project (2021-DST-004) and Youth Innovation Promotion Association, Chinese Academy of Sciences (2023321) .

语种：英文

外文关键词：pesticide diversity; community assembly; network complexity; functional gene; life history strategy

外文摘要：Pesticide application is essential for stabilizing agricultural production. However, the effects of increasing pesticide diversity on soil microbial functions remain unclear, particularly under varying nitrogen (N) fertilizer management practices. In this study, we investigated the stochasticity of soil microbes and multitrophic networks through amplicon sequencing, assessed soil community functions related to carbon (C), N, phosphorus (P), and sulfur (S) cycling, and characterized the dominant bacterial life history strategies via metagenomics along a gradient of increasing pesticide diversity under two N addition levels. Our findings show that higher pesticide diversity enriches the abundance of bacterial specialists and opportunists capable of degrading or resisting pesticides, reducing the proportion of bacterial generalists in the absence of N addition. These shifts can complicate multitrophic microbial networks. Under increased pesticide diversity, selective pressure may drive bacteria to streamline their average genome size to conserve energy while enhancing C, N, P, and S metabolic capacities, thus accelerating soil nutrient loss. In comparison, N addition was found to reduce bacterial niche differentiation at higher pesticide diversity, mitigating the impacts of network complexity and functional traits associated with pesticide diversity, ultimately alleviating soil nutrient loss. Our results reveal the contrasting impacts of pesticide diversity on microbial functions under different N input scenarios and emphasize that strategic N fertilizer management can mitigate the ecological effects of pesticide use in agricultural systems.