文献类型：期刊文献

英文题名：Molecular characterization and expression pattern of an alpha-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii

作者：Ren, G.[1,2];Tang, J. Y.[1];Wang, Y.[1]

机构：[1]Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing, Peoples R China

年份：2014

卷号：13

期号：3

起止页码：6653

外文期刊名：GENETICS AND MOLECULAR RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000343049600030)、、WOS

基金：Research supported by the Special Fund for Agro-Scientific Research in the Public Interest of China (#200903028), Shaoxing Municipal Science and Technology Plan Project of China (#2011A22007), and the Foundation of Zhejiang Education Committee of China (#Y201225243).

语种：英文

外文关键词：Hyriopsis cumingii; alpha-amylase; Gene expression; Food availability

外文摘要：The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of alpha-amylase remains unknown for this species. In this study, the full-length cDNA of the a-amylase gene (HcAmy) was isolated from H. cumingii by the rapid amplification of cDNA ends. Tissue-specific expression analysis showed that HcAmy mRNA was mainly expressed in the hepatopancreas; although, the gene was also expressed in the adductor muscle, intestine, gill, and crystalline style. After 2 weeks starvation, the expression of HcAmy mRNA in the hepatopancreas was upregulated at 24 h after re-feeding or when exposed to algal concentration of 32 mu g/L chlorophyll-a, indicating that the HcAmy mRNA expression in H. cumingii is regulated by algal availability. The results of this study confirm that the HcAmy gene is an important component of the carbohydrate metabolism of H. cumingii fed phytoplankton. In addition, this study demonstrates that the modulation of this gene is dependent on environmental food availability, including starvation, re-feeding time following a period of starvation, and algal concentrations during re-feeding.