文献类型：期刊文献

英文题名：Enhanced production of diosgenin from Dioscorea zingiberensis in mixed culture solid state fermentation with Trichoderma reesei and Aspergillus fumigatus

作者：Cheng, Yutong[1]; Dong, Chun[1]; Huang, Chengcai[1]; Zhu, Yuling[1,2]

机构：[1] College of Life Sciences, Shaoxing University, Shaoxing, China; [2] Key Laboratory of Clean Dyeing and Finishing Technology of Zhejiang Province, School of Chemistry and Chemical Engineering, Shaoxing Universit, Shaoxing, China

年份：2015

卷号：29

期号：4

起止页码：773

外文期刊名：Biotechnology and Biotechnological Equipment

收录：EI(收录号：20152700987686)、Scopus(收录号：2-s2.0-84933511947)

语种：英文

外文关键词：Efficiency - Aspergillus

外文摘要：Diosgenin is a valuable precursor for the synthesis of steroid compounds and is widely used in the pharmaceutical industry. The production of diosgenin from plant tubers by using microbial transformation is a cheap and environmentallyfriendly method. However, the low efficiency of the existing fermentation protocols prevents the largescale production required to meet the industrial demand. In this work, a solid state fermentation of Dioscorea zingiberensis was performed by using a mixed culture of Trichoderma reesei and Aspergillus fumigatus to increase the bioconversion efficiency. We optimized several parameters of the fermentation process of T. reesei and A. fumigatus monocultures using a response surface methodology. Next, we tested fermentation strategies and different ratios of T. reesei:A. fumigatus (T:A) inoculum. A maximum yield (95.82%) resulted from using a T:A ratio of 1:2 (7.5×106 T. reesei spores/g pretreated DZW) and a fermentation strategy, in which T. reesei was added at the start of the fermentation and A. fumigatus was added after 24 hours. ? 2015 The Author(s). Published by Taylor & Francis.