文献类型：期刊文献

英文题名：Homologues of sox8 and sox10 in the orange-spotted grouper Epinephelus coioides: Sequences, expression patterns, and their effects on cyp19a1a promoter activities in vitro

作者：Liu, Qiongyou;Lu, Huijie;Zhang, Lihong;Xie, Jun[2];Shen, Wenying[3];Zhang, Weimin[1]

机构：[1]Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China;[2]Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China;[3]Univ Shaoxing, Sch Life Sci, Shaoxing 312000, Peoples R China

年份：2012

卷号：163

期号：1

起止页码：86

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000307030000009)、、WOS

基金：We thank Guangdong Daya Bay Fishery Development Center (Huizhou City 516081, Guangdong, PR China) for providing the fish embryos and facilities for hatching. This work was supported by National Key Technology Research and Development Program (2012AA10A407, 2008AA09Z406), the Natural Science Foundation of China (330970359, 31072197), the Modern Agro-industry Technology Research System (NYCYTX-49-13), and Zhejiang Provincial Science Foundation Program of China (Y3090171).

语种：英文

外文关键词：Orange-spotted grouper Epinephelus coioides; sox8a; sox10a; cyp19a1a promoter; Transcriptional regulation

外文摘要：Sox8 and Sox10 are members of group E Sox proteins involved in a wide range of developmental processes including sex determination and neurogenesis in vertebrates. The orange-spotted grouper sox8a and sox10a homologues were isolated and characterized in the present study. Both sox8a and sox10a genes contain three exons and two introns, and encode putative proteins with typical structures of group E Sox. Sox8a was expressed in diverse tissues including the central nervous system and some peripheral tissues. In contrast, sox10a mRNA was detected primarily in the central nervous system. During embryogenesis, sox8a mRNA seemed to be de novo synthesized in the embryos from otic vesicle stage. However, sox10a mRNA was only detectable in juvenile fish 35 days post hatching and thereafter. The mRNA levels of sox8a in the gonads were not significantly different among ovarian developmental stages but increased in the testis. In vitro transfection assays showed that the Sox10a but not Sox8a up-regulated cyp19a1a promoter activities. Taken together, these results suggested that the sox8a may play roles in diverse tissues and during embryogenesis, whereas sox10a may be mainly involved in the neural regulation of juvenile and adult fish, and that certain Sox homologues may regulate the orange-spotted grouper cyp19a1a promoter. (C) 2012 Elsevier Inc. All rights reserved.