文献类型：期刊文献

中文题名：C-fos和增殖细胞核抗原在人胎小肠组织中的表达及意义

英文题名：Expression and significance of C-fos and proliferating cell nuclear antigen in the small intestinal tissue of human fetus

作者：刘学红[1];张泳[1]

机构：[1]绍兴文理学院医学院组胚教研室

年份：2011

卷号：36

期号：2

起止页码：145

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD、、Scopus、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：浙江省教育厅科研计划项目(Y200805569);绍兴文理学院重点资助项目(08LG1018)

语种：中文

中文关键词：小肠;增殖细胞核抗原;原癌基因蛋白质c-fos;胚胎;免疫组织化学;原位缺口末端标记

外文关键词：small intestine； proliferating cell nuclear antigen； C-fos protein； Human fetus； Immunohistochemistry； terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling（TUNEL）；

中文摘要：目的探讨增殖细胞核抗原(PCNA)、C-fos蛋白和凋亡因子在人胎小肠组织中的表达规律。方法应用免疫组织化学法和原位末端标记法(TUNEL)检测第2、3、4三个月龄段16例人胎小肠组织细胞增殖和凋亡的表达情况。结果第2～4个月龄段,PCNA和C-fos蛋白在人胎小肠壁组织细胞中均有阳性表达。随着胎龄增加,人胎小肠壁组织细胞中PCNA蛋白的阳性表达数量和染色强度平均值(AI)均呈逐渐升高趋势(P<0.01),C-fos蛋白的阳性表达数量则呈先升高再降低趋势,而AI值呈逐渐升高趋势(P<0.01)。第2～4个月龄段时,TUNEL染色阳性细胞在人胎小肠壁各层组织内均有分布,随着胎龄增加,TUNEL阳性细胞数量和AI值均呈先升高再降低趋势(P<0.01)。结论 PCNA和C-fos蛋白参与人胎早期小肠壁组织细胞的生长发育,尤其在人胎发育第3个月龄段,细胞增殖和凋亡数量明显增多,此期可能是小肠组织发育的关键时期。

外文摘要：Objective To explore the expression rule of proliferating cell nuclear antigen（PCNA）,C-fos proteins and apoptosis genes in the small intestinal tissue of human fetus.Methods At the second-to fourth-month of gestation,the expressions of cell proliferation and apoptosis were observed in 16 specimens of human fetal small intestinal tissue by using the immunohistochemical methods and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling（TUNEL）.Results At the second to fourth month of gestation,all the PCNA and C-fos proteins were positively expressed in the small intestinal tissues and cells of human fetus.With the increase in gestational period,the positive cell number and average intensity（AI） of PCNA protein increased gradually（P〈0.01）.The positive cell number of C-fos protein increased first,and then decreased,while the AI of C-fos protein stably increased in the small intestinal tissues and cells of human fetus（P〈0.01）.At the second to fourth month of gestation,TUNEL positive cells were seen to distribute in each layer of the small intestinal tissues of human fetus.With the increase of age,all the positive cell number and AI of TUNEL positive cells showed a tendency of decrease following increase in the small intestine of human fetus（P〈0.01）.Conclusions PCNA,C-fos and apoptosis gene participate in adjusting the growth and development of the cells and tissues in the small intestine of human fetus.In the third month of gestation,especially,proliferation and apoptosis are significantly increased in the small intestinal tissue of human fetus,which may be the key period of intestinal tissue development.