文献类型：期刊文献

中文题名：南方红豆杉内生真菌的多样性与群落结构

英文题名：Diversity and community structure of endophytic fungi from Taxus chinensis var. mairei

作者：臧威[1];孙翔[2];孙剑秋[1];于文喜[3];王雪松[4];尹军霞[1];宋瑞清[3]

机构：[1]绍兴文理学院生命科学学院;[2]中国科学院微生物研究所真菌学国家重点实验室;[3]黑龙江省林业科学院;[4]齐齐哈尔大学生命科学与农林学院

年份：2014

卷号：25

期号：7

起止页码：2071

中文期刊名：应用生态学报

外文期刊名：Chinese Journal of Applied Ecology

收录：CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014、PubMed

基金：中国科学院微生物研究所真菌学国家重点实验室开放课题(SKLMKF201307);浙江省重大科技专项(2013C02006-1);黑龙江省自然科学基金项目(C200806)资助

语种：中文

中文关键词：内生真菌;多样性;群落结构;南方红豆杉;ITS

外文关键词：endophytic fungi ; diversity; community structure ; Taxus chinensis var. mairei; ITS.

中文摘要：从南方红豆杉480个针叶与枝条组织块中分离到628株内生真菌,根据形态特征和ITS序列鉴定为28属43个分类单元.其中,包括10种丝孢菌、20种腔孢菌、12种子囊菌和1种未知真菌,茎生拟茎点霉为优势种;根据相对频率,链格孢、出芽短梗霉、博宁刺盘孢、盘长孢状刺盘孢、黑附球菌、Fungal sp.、砖红镰孢、围小丛壳、Magnaporthales sp.、稻黑孢、斑点拟盘多毛孢、小孢拟盘多毛孢、Peyronellaea glomerata和Xylaria sp.1等在南方红豆杉中比较常见.南方红豆杉受到内生真菌侵染的程度较高,81%的组织有内生真菌存在,其资源丰富并具有较高的物种多样性;内生真菌分布受组织性质影响,南方红豆杉针叶中内生真菌的定植率、丰富度、多样性明显低于枝条,枝条间内生真菌组成比针叶与枝条间更加相似,表现出组织偏好性或专一性;组织年龄影响内生真菌的群落结构,随着南方红豆杉枝条组织年龄的增长,内生真菌的定植率、丰富度、多样性均表现出逐渐增加的趋势.系统研究南方红豆杉内生真菌的多样性与群落结构,阐明内生真菌在植物组织中的分布规律,可以为南方红豆杉内生真菌的开发利用提供基础资料和科学依据;探讨病原真菌在健康植物组织中的存在情况,对于南方红豆杉的植物资源保护具有积极意义.

外文摘要：A total of 628 endophytic fungi were isolated from 480 tissue segments of needles and branches of Taxus chinensis var. mairei. According to morphological characteristics and ITS se- quences, they represented 43 taxa in 28 genera, of which 10 Hyphomycetes, 20 Coelomycetes, 12 Ascomycetes and 1 unknown fungus. Phomopsis mall was confirmed as the dominant species. In ac- cordance with relative frequency, Alternaria alternata, Aureobasidium pullulans, Colletotrichum boninense, C. gloeosporioides, Epicoccum nigrum, Fungal sp., Fusarium lateritium, Glomerella cingulata , Magnaporthales sp. , Nigrospora oryzae , Pestalotiopsis maculiformans , P. microspora , Peyronellaea glomerata and Xylaria sp. 1 were more common in T. chinensis var. mairei. T. chinensis var. mairei were severely infected by endophytic fungi. Endophytic fungi were found in 81 percent of plant tissues with a high diversity. Distribution ranges of endophytic fungi were influ- enced by tissue properties. The colonization rate, richness, diversity of endophytic fungi in needles were obviously lower than in branches, and kinds of endophytic fungi between branches were more similar than those in needles, thus endophytic fungi had tissue preference. In addition, tissue age influenced the community structure of endophytic fungi. The elder branch tissues were, the higher colonization rate, richness, diversity of endophytic fungi were. Systematic studying the diversity and community structure of endophytic fungi in T. chinensis var. mairei and clarifying their distribution regularity in plant tissues would offer basic data and scientific basis for their development and utili- zation. Discussing the presence of fungal path nificance for source protection of T. chinensis ogens in healthy plant tissues would be of positive sig- var. mairei.