文献类型：期刊文献

中文题名：姜黄素衍生物B06对2型糖尿病大鼠肝脏的保护作用

英文题名：Effect of curcumin derivative B06 on liver from type 2 diabetic rats

作者：王玲[1];徐梦菲[1];刘曦[2];王维维[3];陈三妹[4];程锦国[2];陈国荣[1]

机构：[1]温州医科大学附属第一医院病理科;[2]温州医科大学附属第二医院;[3]浙江大学附属第二医院病理科;[4]绍兴文理学院医学院

年份：2014

卷号：30

期号：2

起止页码：328

中文期刊名：中国病理生理杂志

外文期刊名：Chinese Journal of Pathophysiology

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：浙江省公益性技术应用研究计划项目(No.2011c23123);温州市高层次人才创新技术项目

语种：中文

中文关键词：糖尿病;高脂血症;姜黄素衍生物B06;肝;AMP活化蛋白激酶仪;大鼠

外文关键词：Diabetes mellitus ; Hyperlipidemia; Curcumin derivative B06 ; Liver; AMP-activated protein ki- nase α; Rats

中文摘要：目的:研究姜黄素衍生物B06对2型糖尿病大鼠肝脏的保护作用及机制。方法:雄性SD大鼠35只,随机均分成5组:正常对照组、高脂组、高脂治疗组、糖尿病组和糖尿病治疗组。采用高脂饮食加链脲佐菌素诱导2型糖尿病大鼠模型。高脂治疗组及糖尿病治疗组用0.2 mg·kg-1·d-1B06灌胃8周。治疗结束后用光镜和透射电镜观察大鼠肝组织的形态学改变,并用Western blotting方法检测肝脏AMP活化蛋白激酶α(AMPKα)和磷酸化AMPKα(p-AMPKα)蛋白的表达。结果:高脂组及糖尿病组大鼠肝脏显示肝细胞脂肪变性、坏死,炎症细胞浸润,纤维组织增生,2型糖尿病大鼠和高脂血症大鼠肝脏组织p-AMPKα表达降低;经B06干预后,糖尿病大鼠和高脂血症大鼠肝脏的形态学损害明显得到改善,且肝脏组织p-AMPKα的表达水平升高(P<0.05)。结论:B06对2型糖尿病大鼠的肝脏具有保护作用,可能与其上调肝脏p-AMPKα蛋白表达有关。

外文摘要：AIM ： To observe the protective effect of curcumin derivative B06 on the liver from the rats with hy- perlipidemia and type 2 diabetes mellitus. METHODS ： Male Sprague-Dawley rats （ n = 35 ） were divided randomly into 5 groups： normal control group, high-fat group, high-fat ＋ B06-treated group, diabetic group and diabetic ＋ B06-treated group. After fed with a high-fat diet for 4 weeks, the rats in the later 2 groups were injected with streptozotocin intraperito- neally to induce type 2 diabetes mellitus. The rats in B06-treated groups were given B06 by gavage at a dose of 0.2 mg · kg ^- 1·d^ - 1 for 8 weeks. After the treatment, the morphology of the liver was observed under light and transmission electron microscopes. The protein expression of AMP-activated protein kinase α （AMPKα） and phosphorylated AMPK α （p- AMPKα） was detected by Western blotting. RESULTS： Fatty degeneration, hepatocellular necrosis, inflammatory cell in- filtration and hyperplasia of fibrous tissue were observed in the liver from the rats in high-fat group and diabetic group, and were relieved after B06 treatment. The protein expression of p-AMPKα was decreased in the liver of the rats in diabetic group and high-fat group, and it was increased in the liver of the high-fat and diabetic rats in B06-treated group. CON- CLUSION： Cureumin derivative B06 exerts a protective effect on the liver in type 2 diabetic rats, and the increased ex- pression of p-AMPKα may be involved in the mechanism of protection.