文献类型：会议论文

英文题名：Simultaneous determination of atractylenolide II and III in rhizoma atractylodes macrocephalae and Chinese medicinal preparation by reverse-phase high-performance liquid chromatography

作者：Sun, Xiao-Hong[1]; Ge, Jian[2]

机构：[1] Shaoxing University, Yuanpei College, Shaoxing 312000, China; [2] College of Life Science, China Jiliang University, #258 XueYuan Street, XiaSha Higher Education Zone, Hangzhou 310018, Zhejiang Province, China

会议论文集：Frontier and Future Development of Information Technology in Medicine and Education, ITME 2013

会议日期：July 19, 2013 - July 21, 2013

会议地点：Xining, China

语种：英文

外文关键词：Medicine - Organic solvents

外文摘要：This present paper describes the development of a reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry for simultaneous determination of atractylenolide II and III in traditional Chinese medicines and Chinese medicinal preparation. The standard of atractylenolide II and III was isolated from Rhizoma Atractylodes Macrocephalae. From a variety of solvents tested, methanol was found to be the best solvent for simultaneously extracting atractylenolide II and III from Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation samples. RP-HPLC analysis of the extracts was performed on an analytical column (SHIMADZU ODS, 150 × 4.6 mm; i.d., 5 lm) equipped with a security guard pre-column system. The results showed that there was a good linearity over the range 0.5-50.0 lg/ml (r > 0.99). The recoveries were more than 95.0 % in Rhizoma Atractylodes Macrocephalae, and the intra- and inter-day coefficients of variation were less than 5.0 % in all cases. The lower limit of quantification (LLOQ) was 0.5 lg/ml. The RP-HPLC method was readily applied to simultaneously quantitate atractylenolide II and III in Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation (Shenlingbaizhu granule). ? Springer Science+Business Media Dordrecht 2014.