文献类型：期刊文献

中文题名：自制移植肝低温保存液对氧自由基的影响

英文题名：Effects of self-made cryopreservation solution on oxygen free radicals in cryopreserved rat liver

作者：王露佳[1];孙雯[1];张金萍[1];王超[1];赵小瑜[1];朱玲颖[1];马静茹[1];唐雀美[1];郑丹燕[1]

机构：[1]绍兴文理学院医学院临床医学系

年份：2013

期号：5

起止页码：837

中文期刊名：中国组织工程研究

外文期刊名：Chinese Journal of Tissue Engineering Research

收录：CSTPCD、、CSCD_E2013_2014、Scopus、CSCD

基金：浙江省大学生科技创新活动计划暨新苗人才计划(2012R426025);绍兴文理学院医学院学生科研项目(201106);绍兴文理学院第一批校级优秀教学团队建设成果(200907)~~

语种：中文

中文关键词：器官移植;肝移植;器官保存液;自制器官保存液;UW液;低温保存;肝脏;移植;氧自由基;省级基金

外文关键词：organ transplantation; liver transplantation; organ preservation solution; self-made organ preservation solution; UW solution; cryopreservation; liver; transplantation; oxygen free radicals; provincial grants-supported paper

中文摘要：背景:在肝脏移植过程中,低温保存和缺血可导致肝脏产生氧自由基而损伤肝组织。目的:研究自制器官保存液对低温保存大鼠肝脏氧自由基的影响,并与器官保存液的"金标准"UW液进行对比。方法:16只9周龄SD大鼠随机分为实验组和对照组,每组8只。建立SD大鼠肝脏灌注模型,分别用自制器官保存液组和UW液组灌洗肝脏,取出肝脏置于4℃保存液中,于保存24,48,72h后分别检测肝脏超氧化物歧化酶、一氧化氮合酶、总抗氧化能力和活性丙二醛水平。结果与结论:自制器官保存液对大鼠肝脏低温保存各时点超氧化物歧化酶、一氧化氮合酶、总抗氧化能力活性及丙二醛含量与UW液组比较差异均无显著性意义(P>0.05),表明自制器官保存液能够减轻缺血再灌注后氧自由基对大鼠肝脏的损伤。自制器官保存液对低温保存大鼠肝脏在减轻氧自由基损伤方面有较好的效果,与UW液相当。

外文摘要：BACKGROUND： During liver transplantation, cryopreservation and ischemia can lead to production of oxygen free radicals by the liver which damages liver tissue. OBJECTIVE： To study the effects of self-made cryopreservation solution on oxygen free radicals in cryopreserved rat liver, and to compare with ＂gold standard＂ organ preservation solution-UW solution. METHODS： Sixteen 9-week-old Sprague Dawley rats were randomly divided into two groups： experimental group and control group, 8 rats in each group. Sprague Dawley rat liver perfusion models were established, and the livers in each group were lavaged with self-made organ preservation solution and UW preservation solution, then removed the liver and placed into 4℃preservation solution. The superoxide dismutase, nitric oxide synthase, total antioxidant capacity activity and the malondialdehyde content were detected at 24, 48 and 72 hours after preservation. RESULTS AND CONCLUSION： There were no significant differences in superoxide dismutase, nitric oxide synthase, total antioxidant capacity activity and the malondialdehyde content in rat liver tissue cryopreserved in the self-made organ preservation solution versus UW solution （P 0.05）. These findings suggest that self-made organ preservation solution can reduce the damage of oxygen free radical to the livers of rats after ischemia-reperfusion and the effect is equivalent to UW solution.