文献类型：期刊文献

英文题名：A non-canonical role of ATG8 in Golgi recovery from heat stress in plants

作者：Zhou, Jun[1,2];Ma, Juncai[3,4];Yang, Chao[1,2,5,6];Zhu, Xiu[1,2];Li, Jing[3,4];Zheng, Xuanang[1,2];Li, Xibao[1,2];Chen, Siyu[1,2];Feng, Lei[1,2,3,4];Wang, Pengfei[3,4];Ho, Man Ip[3,4];Ma, Wenlong[3,4];Liao, Jun[1,2];Li, Faqiang[7];Wang, Chao[8];Zhuang, Xiaohong[3,4];Jiang, Liwen[3,4];Kang, Byung-Ho[3,4];Gao, Caiji[1,2]

机构：[1]South China Normal Univ, MOE Key Lab, Guangzhou Key Lab Spectral Anal & Funct Probes, Coll Biophoton,Guangdong Prov Key Lab Biotechnol P, Guangzhou, Peoples R China;[2]South China Normal Univ, Guangdong Prov Key Lab Laser Life Sci, Guangzhou Key Lab Spectral Anal & Funct Probes, Coll Biophoton,Guangdong Prov Key Lab Biotechnol P, Guangzhou, Peoples R China;[3]Chinese Univ Hong Kong, Ctr Cell & Dev Biol, Sch Life Sci, Hong Kong, Peoples R China;[4]Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China;[5]Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou, Peoples R China;[6]Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Imp, South China Bot Garden, Guangzhou, Peoples R China;[7]South China Agr Univ, Coll Life Sci, Guangzhou, Peoples R China;[8]Shaoxing Univ, Coll Life Sci, Shaoxing, Peoples R China

年份：2023

外文期刊名：NATURE PLANTS

收录：SCI-EXPANDED(收录号：WOS:000972833000001)、、Scopus(收录号：2-s2.0-85153338026)、WOS

基金：This work was supported by grants from the National Natural Science Foundation of China (grant nos. 32061160467, 32270291 and 31870171) and Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China (grant no. 171014) to C.G., the National Science Foundation of China (grant no. 31600288) and the Basic Research Program of Guangzhou (grant no. 202201010508) to J.Z., and Hong Kong Research Grant Council (grant nos. GRF14113921, GRF14109222, N_CUHK462/22 and C4002-20W) to B.-H.K. We acknowledge the support of the 2022 Guangdong-Hong Kong-Macao Greater Bay Area Exchange Programs of SCNU.

语种：英文

外文摘要：Above-optimal growth temperatures, usually referred to as heat stress (HS), pose a challenge to organisms' survival as they interfere with essential physiological functions and disrupt cellular organization. Previous studies have elucidated the complex transcriptional regulatory networks involved in plant HS responses, but the mechanisms of organellar remodelling and homeostasis during plant HS adaptations remain elusive. Here we report a non-canonical function of ATG8 in regulating the restoration of plant Golgi damaged by HS. Short-term acute HS causes vacuolation of the Golgi apparatus and translocation of ATG8 to the dilated Golgi membrane. The inactivation of the ATG conjugation system, but not of the upstream autophagic initiators, abolishes the targeting of ATG8 to the swollen Golgi, causing a delay in Golgi recovery after HS. Using TurboID-based proximity labelling, we identified CLATHRIN LIGHT CHAIN 2 (CLC2) as an interacting partner of ATG8 via the AIM-LDS interface. CLC2 is recruited to the cisternal membrane by ATG8 to facilitate Golgi reassembly. Collectively, our study reveals a hitherto unanticipated process of Golgi stack recovery from HS in plant cells and uncovers a previously unknown mechanism of organelle resilience involving ATG8. This study reveals that short-term acute heat stress causes vacuolation of the Golgi apparatus, whereafter autophagy-related protein 8 (ATG8) translocates to the cisternal membrane and recruits CLATHRIN LIGHT CHAIN 2 (CLC2) to facilitate Golgi reconstruction by promoting the budding of ATG8-positive vesicles.