文献类型：期刊文献

英文题名：Improved Catalytic Performance of Lipase Supported on Clay/Chitosan Composite Beads

作者：Tu, Ni[1];Shou, Jianxin[2];Dong, Huaping[1];Huang, Jin[1];Li, Yimin[1]

机构：[1]Shaoxing Univ, Coll Chem & Chem Engn, Huancheng West Rd 508, Shaoxing 312000, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Huancheng West Rd 508, Shaoxing 312000, Peoples R China

年份：2017

卷号：7

期号：10

外文期刊名：CATALYSTS

收录：SCI-EXPANDED(收录号：WOS:000414596300022)、、Scopus(收录号：2-s2.0-85032587246)、WOS

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 21477081, 21177088, 21677101); the Natural Science Foundation of Zhejiang Province, China (Grant No. LY16B070004); and the Research Project for Public Welfare of Zhejiang Province, China (Grant No. 2015C33229).

语种：英文

外文关键词：lipase; immobilization; clay/chitosan composite; hydrolysis; catalytic performance

外文摘要：Clay/chitosan composite beads were prepared and used as the carrier to support lipase by adsorption, to improve the activity and stability of lipase in the hydrolysis of olive oil. Under conditions of pH 6.0, 25 degrees C and adsorption for 10 h, immobilized lipases on chitosan bead (CB-lipase) and three clay/chitosan composite beads, at different clay to chitosan proportions of 1:8 (CCB-8-lipase), 1:5 (CCB-5-lipase) and 1:3 (CCB-3-lipase), were prepared. By comparing the activity of these immobilized lipases, CCB-5-lipase showed the highest activity, followed by CCB-8-lipase > CCB-3-lipase > CB-lipase; this improvement was attributed to the synergetic effect of enrichment of olive oil by clay at the reaction surface and better biocompatibility of chitosan with lipase molecules. The optimum pH and temperature in the reaction respectively changed from 7.0 and 30 degrees C for free lipase to 7.5 and 35 degrees C for immobilized forms. Furthermore, the thermal stability and repeated usability of these immobilized lipases were sequenced as CCB-3-lipase > CCB-5-lipase > CCB-8-lipase > CB-lipase, due to greater rigidity of immobilized lipase with the addition of clay, which was further confirmed by SEM. The study shows that the incorporation of clay with chitosan creates a good synergetic effect to improve the catalytic performance of immobilized lipase on clay/chitosan composite.