文献类型：期刊文献

中文题名：体外MPP^+诱导胚胎大鼠中脑多巴胺能神经元模型的建立

英文题名：Establishment of the model of embryonic rat mesencephalic dopaminergic neurons induced by MPP^+

作者：龚超超[1];郎娟[2];熊中奎[1]

机构：[1]绍兴文理学院医学院临床医学部;[2]杭州师范大学基础医学部

年份：2012

卷号：52

期号：43

起止页码：17

中文期刊名：山东医药

外文期刊名：Shandong Medical Journal

收录：CSTPCD

基金：浙江省自然科学基金资助项目(Y2100035);浙江省公益性技术应用研究计划项目(2010C33166);浙江省教育厅高校科研计划项目(Y201018615)

语种：中文

中文关键词：多巴胺神经元;大鼠;中脑;细胞模型;1-甲基-4-苯基-l,2,3,6-四氢吡啶离子

外文关键词：dopaminergic neuron ; rat ; mesencephalon; cell model ; 1 -methyl-4-phenyl-1,2,3,6-tetrahydropyridine

中文摘要：目的建立一种体外1-甲基-4-苯基-l,2,3,6-四氢吡啶离子(MPP+)诱导的胚胎大鼠中脑多巴胺(DA)能神经元模型,为帕金森病(PD)发病机制和药物干预研究奠定基础。方法体外培养胚胎SD大鼠(孕14～15 d)中脑DA神经元7 d后随机分为实验组和对照组,均以含1%B27的无血清DMEM/F12培养基培养,在此基础上前者加入MPP+使终浓度达到10μmol/L,均继续培养48 h。采用免疫组化染色法检测酪氨酸羟化酶(TH)阳性神经元数量和突起长度。结果在放大倍数(×100)视野下,实验组和对照组中脑TH阳性神经元数量分别为(351.5±32.7)、(538.0±58.7)个,P<0.01;在放大倍数(×200)视野下,实验组和对照组中脑TH阳性神经元突起长度分别为(121.6±6.1)、(246.9±11.3)μm(P<0.001)。结论 MPP+体外诱导可成功建立胚胎大鼠中脑DA能神经元模型,此为PD发病机制和药物干预研究奠定了基础。

外文摘要：Objective To establish a cultured cell model of embryonic rat mesencephalic dopaminergic （DA） neurons induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（ MPP＋ ）, and to lay foundation for the study of pathogenesis and drug intervention of Parkinson disease （PD）. Methods The embryonic rat （E14-15） mesencephalic dopaminergic new rons cultured in vitro for 7 days were divided into experimental group and control group, and then were both cultured with serum-free DMEM/F12 including 1% B27, the former was induced by 10 μmol/L of MPP＋ at the same time for 48 hours. The number of tyrosine hydroxylase（TH） positive neurons and neurite length were determined by immuuocytochemical ex- amination. Results Under the magnification field of 100 times ,TH （ ＋ ） neurons was 538.0 ± 58.7 in control group, and 351.5 ± 32.7 in experimental group, P 〈 O. O1 ; Under the magnification field of 200 times, the neurite length was （246.9 ± 11.3） μm in control group, and （121.6 ±6.1 ）μm in MPP＋ group（P〈0. O01）. Conclusions The model of embry- onic rat meseneephalic dopaminergie neurons can be successfully induced by MPP＋ , which could be a reliable means for latter study on pathological mechanism and drug intervention for PD.