文献类型：期刊文献

中文题名：微柱凝胶离心-HPLC测定酮康唑醇质体包封率

英文题名：Determination of Entrapment Efficiency of Ketoconazole Ethosomes with Dextarn Gel Minicolumn Centrifugation-HPLC

作者：王素芳[1];刘利萍[1];边可君[2]

机构：[1]浙江万里学院生物与环境学院;[2]绍兴文理学院药学系

年份：2011

卷号：28

期号：6

起止页码：527

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD、、北大核心2008、CSCD_E2011_2012、北大核心、CSCD

基金：绍兴市科技局重点资助项目(2008A23017)

语种：中文

中文关键词：酮康唑醇质体;包封率;微柱凝胶离心法;高效液相色谱法

外文关键词：ketoconazole ethosomes; entrapment efficiency; minicolumn gel centrifugation; HPLC

中文摘要：目的建立酮康唑醇质体包封率的微柱凝胶离心-HPLC测定方法。方法药物含量采用HPLC测定,醇质体与游离药物的分离采用葡聚糖凝胶微柱离心法。色谱条件为:色谱柱Sinochrom ODS-BP(4.6 mm×200 mm,5μm),流动相乙腈-水-三乙胺(60∶40∶0.5,磷酸调pH 8.6),流速1.0 mL.min-1,检测波长243 nm,进样量20μL。分离条件为:以2 000 r.min-1离心3 min制备葡聚糖凝胶微柱,测样量0.2 mL,以2 400 r.min-1离心5 min,再用0.2 mL蒸馏水洗脱,重复3次。结果微柱对酮康唑的平均吸附率>99.30%,对空白醇质体洗脱率>99.49%。结论葡聚糖凝胶微柱离心-HPLC可作为醇质体包封率测定的简单快捷方法。

外文摘要：OBJECTIVE To develop a determination method for the entrapment efficiency of ketoconazole ethosomes.METHODS The content of ketoconazole was determined by HPLC.The minicolumn of filling dextran gel was adopted to separate the free ketoconazole from ethosome dispersions by centrifugation.Chromatographic conditions were as follows： column was Sinochrom ODS-BP（4.6 mm×200 mm,5 μm） and the mobile phase was acetonitrile-water-triethylamine（60∶40∶0.5,adjust to pH 8.6 using phosphate） with the flow rate of 1.0 mL.min-1,and detection wavelength was 243 nm.The Dextran G-50 minicolumn was spinned at 2 000 r.min-1 for 3 min for removing excess amount of water.The 0.2 mL sample added on prepared column was centrifuged at 2 400 r.min-1 for 5 min,and then washed for 3 times by 0.2 mL of distilled water.RESULTS By flowing through the Dextran G50 minicolumn,the free ketoconazole was adsorbed more than 99.30%,while the average recovery of blank ethosomes was more than 99.49%.CONCLUSION This method is technically simple and rapid to determine entrapment efficiency of ketoconazole ethosomes.