文献类型：期刊文献

英文题名：Overexpression of the Tibetan Plateau annual wild barley (Hordeum spontaneum) HsCIPKs enhances rice tolerance to heavy metal toxicities and other abiotic stresses

作者：Pan, Weihuai[1,2];Shen, Jinqiu[3];Zheng, Zhongzhong[3];Yan, Xu[1];Shou, Jianxin[2];Wang, Wenxiang[3];Jiang, Lixi[4];Pan, Jianwei[1]

机构：[1]Lanzhou Univ, Sch Life Sci, MOE Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[3]Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China;[4]Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Hangzhou 310058, Zhejiang, Peoples R China

年份：2018

卷号：11

期号：1

外文期刊名：RICE

收录：SCI-EXPANDED(收录号：WOS:000444819400001)、、WOS

基金：This study was supported by the National Science and Technology Major Program for New Varieties of Genetically Modified Crops (2009ZX08009076-003), the National Natural Science Foundation of China (No. 31670283 and 91754104), the Natural Science Foundation of Zhejiang Province (No. LY16C130004 and LY17C060001), and the Fundamental Research Funds for the Central Universities (lzujbky-2017-ot16).

语种：英文

外文关键词：Abiotic stresses; Cloning; Heavy metal toxicity; HsCIPKs; Overexpression; Tibetan plateau annual wild barley; Rice; Transformation

外文摘要：Background: The calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) signaling system plays a key regulatory role in plant stress signaling. The roles of plant-specific CIPKs, essential for CBL-CIPK functions, in the response to various abiotic stresses have been extensively studied so far. However, until now, the possible roles of the CIPKs in the plant response to heavy metal toxicities are largely unknown. Results: In this study, we used bioinformatic and molecular strategies to isolate 12 HsCIPK genes in Tibetan Plateau annual wild barley (Hordeum spontaneum C. Koch) and subsequently identified their functional roles in the response to heavy metal toxicities. The results showed that multiple HsCIPKs were transcriptionally regulated by heavy metal toxicities (e.g., Hg, Cd, Cr, Pb, and Cu) and other abiotic stresses (e.g., salt drought, aluminum, low and high temperature, and abscisic acid). Furthermore, the ectopic overexpression of each HsCIPK in rice (Oryza sativa L cv Nipponbare) showed that transgenic plants of multiple HsCIPKs displayed enhanced tolerance of root growth to heavy metal toxicities (Hg, Cd, Cr, and Cu), salt and drought stresses. These results suggest that HsCIPKs are involved in the response to heavy metal toxicities and other abiotic stresses. Conclusions: Tibetan Plateau annual wild barley HsCIPKs possess broad applications in genetically engineering of rice with tolerance to heavy metal toxicities and other abiotic stresses.