文献类型：期刊文献

中文题名：银鲫消化酶的研究

英文题名：Study on digestive enzymes of Carassius auratus glibelio

作者：沈文英[1];寿建昕[1];金叶飞[1];祝尧荣[1];钱伟平[1]

机构：[1]绍兴文理学院生物系

年份：2002

卷号：11

期号：3

起止页码：193

中文期刊名：上海水产大学学报

外文期刊名：Journal of Shanghai Fisheries University

收录：CSTPCD、、CSCD_E2011_2012、CSCD

语种：中文

中文关键词：银鲫;消化酶;活性分布;pH值;酶活性;生长阶段;器官

外文关键词：Carassius auratus glibelio; digestive enzyme; activity distribution; pH value

中文摘要：对处于不同生长阶段银鲫的蛋白酶、脂肪酶、淀粉酶、纤维素酶的活性分布进行了初步研究。结果表明 :在鱼苗、鱼种、幼鱼、成鱼和亲鱼 5个生长阶段 ,胰蛋白酶和脂肪酶活性基本保持不变 ,纤维素酶活性呈上升趋势 ,淀粉酶活性从鱼苗到成鱼不断提高 ,但在亲鱼阶段降低。胰蛋白酶和淀粉酶活性分布均以中肠最大 ,纤维素酶活性则在肝胰脏中最大。在肝胰脏、前肠、中肠、后肠 ,胰蛋白酶的最适pH值分别是 7.5、7.5、8.5、8.0 ;脂肪酶的最适pH值均为 7.5。淀粉酶的最适pH值均为 6 .5 ;纤维素酶在肝胰脏的最适pH值是 4.8,在前肠、中肠、后肠最适pH值均有两个峰值 ,分别为 3.6和 4.8。

外文摘要：The activity distribution of trypsin, lipase,amylase and cellulase in Carassius auratus glibelio was examined. The activity of cellulase was increased, and those of lipase and trypsin remained at similar levels during fry, fingerling, juvenile, mature and reproductive stages. The amylase activity was increased from fry to mature stages then decreased at reproductive stage. The activities of trypsin and amylase are higher in intestine sectionⅡ and the cellullase activity is higher in hepatopancreas. The optimum pH values of hepatopancreas ,foregut,midgut,hindgut for the trypsin are 7.5,7.5,8.5,8.0 respectively, and for the lipase are 7.5,and for the amylase are 6.5. The optimum pH value of hepatopancreas for the cellulase is 4.8, and there are two optimum pH values of intestine for the cellulase 3.6 and 4.8. These results showed that different pH values were conductive to different digestive enzymes.