文献类型：期刊文献

英文题名：Involvement of COX-1 and up-regulated prostaglandin E synthases in phosphatidylserine liposome-induced prostaglandin E-2 production by microglia

作者：Zhang J.[1,2];Fujii S.[1,5];Wu Z.[1];Hashioka S.[1];Tanaka Y.[3];Shiratsuchi A.[4];Nakanishi Y.[4];Nakanishi H.[1]

机构：[1]Kyushu Univ, Fac Dent Sci, Lab Oral Aging Sci, Fukuoka 8128582, Japan;[2]Shaoxing Univ, Coll Med, Shaoxing, Peoples R China;[3]Kyushu Univ, Grad Sch Pharmaceut Sci, Div Pharmaceut Cell Biol, Fukuoka 8128582, Japan;[4]Kanazawa Univ, Grad Sch Med Sci, Kanazawa, Ishikawa 9200934, Japan

年份：2006

卷号：172

期号：1-2

起止页码：112

外文期刊名：JOURNAL OF NEUROIMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000236266900012)、、Scopus(收录号：2-s2.0-33244479399)、WOS

基金：The authors thank Ms. Misao Sakamoto (Kyushu University) for her excellent technical assistance with preparation of primary cultured microglia. This study was supported by Grants-in-Aid for the Creation of Innovations through Business–Academic–Public Sector Cooperation of Japan, and Grants-in-Aid for Scientific Research on Priority Area (No. 15082204) from the Ministry of Education, Science and Culture, Japan.

语种：英文

外文关键词：phosphatidylserine liposome; phagosytosis; class B scavenger receptor type I; prostaglandin E-2; microglia; cyclooxygenase-1; cyclooxygenase-2; cytosolic prostaglandin E synthase; membrane-bound prostaglandin E synthase-2; phosphatidylserine-specific receptor; p44/p42 extracellular signal-regulated kinase

外文摘要：After engulfment of apoptotic cells through phosphatidylserine (PS)-mediated recognition, microglia secrete prostaglandin E-2 (PGE(2)), a potent anti-inflammatory molecule in the central nervous system. Despite the clinical significance, the mechanism underlying PGE(2) production by phagocytosis of apoptotic cells is poorly understood. In the present study, we used PS liposomes to elucidate the phagocytic pathway for PGE(2) production in microglia, because PS liposomes mimic the effects of apoptotic cells on microglia/macrophages. The level of PGE(2) in the culture medium of primary cultured rat microglia was significantly increased by PS liposomes treatment but not by phosphatidylcholine liposomes treatment. The specific ligand for class B scavenger receptor (SR-B), high density lipoprotein, significantly suppressed PS liposome-induced PGE(2) production. PS liposomes were immediately phagocytosed by microglia and sorted to endosomes/lysosomes. Cyclooxygenase (COX)-2 and membrane-bound prostaglandin E synthase-1 (mPGES-1) were induced by treatment with lipopolysaccharide (LPS) but not with PS liposomes. On the other hand, mPGES-2 and cytosolic PGES (cPGES) that are functionally coupled with COX-1 were upregulated after treatment with PS liposomes or LPS. Furthermore, PS liposome-induced PGE(2) production was significantly suppressed by indomethacin, a preferential COX-1 inhibitor, but not by NS-398, a selective COX-2 inhibitor. PS liposomes induced activation of p44/p42 extracellular signal-regulated kinase (ERK) but not p38 mitogen-activated protein kinase in SR-BI independent manner. These observations strongly suggest that the up-regulation of terminal PGESs that are preferentially coupled with COX-1, especially mPGES-2, plays the pivotal role in PS liposome-induced PGE(2) production by microglia. Although SR-BI plays an essential role in PS liposome-induced PGE(2) production, other PS-recognizing receptors, possibly PS-specific receptor, could also promote PGE(2) production by transducing intracellular signals including p44/p42 ERK after PS liposomes treatment. (C) 2005 Elsevier B.V. All rights reserved.