文献类型：期刊文献

英文题名：Immunoreactivity Analysis of the Nonstructural Proteins of Human Enterovirus 71

作者：Chen, Xuyan[1];Qin, Bo[2];Shi, Min[1];Zhu, Longying[1];Sun, Menglin[1];Liu, Xufeng[1];Zhang, Jianhua[1]

机构：[1]Shaoxing Univ, Sch Med, Dept Lab Med, 900 Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Peoples Hosp, Clin Lab Ctr, Shaoxing, Zhejiang, Peoples R China

年份：2017

卷号：30

期号：2

起止页码：106

外文期刊名：VIRAL IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000395588500004)、、Scopus(收录号：2-s2.0-85019004305)、WOS

基金：The authors are grateful to Dr. Jihong Meng (Southeast University, People's Republic of China) for his valuable suggestions and help to Dr. Bo Zhang (Chinese Academy of Science, People's Republic of China) for the gift of infectious clones of EV-A71. This work was supported by the grants from the Natural Science Foundation of Zhejiang Province (grant no. LY16H190005) and the Undergraduate Scientific and Technological Innovation Project of Zhejiang Province (grant no. 2014R426033).

语种：英文

外文关键词：enterovirus 71; nonstructural protein; immunoreactivity; antibody response; hand; foot; mouth disease

外文摘要：Human enterovirus 71 (EV-A71) is one of the main etiological agents of hand, foot, and mouth disease (HFMD), which has been prevalent mainly in the Asia-Pacific region in the past several decades. The nonstructural proteins of EV-A71 will be expressed significantly during viral replication in host cells after EV-A71 infection. For the determination of the antibodies response against nonstructural proteins of EV-A71, in this study, the complete 2ABC, 3ABC, and 3D proteins were expressed in Escherichia coli and were then studied for their immunoreactivity by immunoblot assay and indirect enzyme-linked immunosorbent assay (ELISA), respectively. Three His-tagged fusion proteins were expressed effectively in E. coli, which were in agreement with the expected molecular mass. The results from immunoblot assay and indirect ELISA showed that all three purified fusion proteins can react with IgG antibodies from EV-A71-infected patients, but can hardly be recognized by IgG antibodies derived from mice or rabbits immunized by inactivated EV-A71 virus particles. The IgG antibody response against nonstructural proteins of EV-A71 is associated with viral infection or replication, which indicate that these nonstructural proteins could be used as candidate antigen for early diagnosis of EV-A71 infection, or to distinguish the EV-A71-specific antibodies after viral infection from inactivated vaccine immunization.