文献类型：期刊文献

中文题名：OsPIN1a基因在水稻植株不同组织表达与定位研究

英文题名：Research on Expression and Localization of OsPIN1a Gene in Different Tissues in Rice

作者：赵亚波[1];卜贤盼[1];李培纲[1];王路蝉[1];陈晶晶[2];莫亿伟[1,2]

机构：[1]绍兴文理学院生命科学学院;[2]中国热带农业科学院南亚热带作物研究所

年份：2014

卷号：24

期号：1

起止页码：27

中文期刊名：生物技术

外文期刊名：Biotechnology

收录：北大核心2011、CSCD_E2013_2014、北大核心、CSCD

基金：国家自然科学基金项目( "生长素极性运输与钙信号对水稻根负向光性影响";No. 31071353);绍兴文理学院科研启动经费( "OsPIN1a基因在水稻根生长 及 负 向 光 性 中 的 作 用 研 究 ";No. 20135004) 资助~~

语种：中文

中文关键词：水稻;OsPIN1a;生长素极性运输;绿色荧光蛋白;GUS染色;根尖

外文关键词：Rice ; OsPIN1a; Polar auxin transport; GFP; GUS staining; Root tip

中文摘要：目的:为了探讨水稻生长素极性运输输出载体蛋白OsPIN1a的在水稻不同组织的作用和分布。方法:以带有GFP和GUS标记的转OsPIN1a基因水稻和野生型中花11水稻为研究材料。通过PCR方法检测抗性标记基因潮霉素基因判断植株是否阳性;通过半定量RT-PCR方法分析OsPIN1a基因在转基因植株幼穗、叶片、根系的表达;通过GUS组织染色检测OsPIN1a在幼穗、叶片、根系中的活性;通过激光共聚焦显微镜观察OsPIN1a-GFP蛋白在根尖亚细胞定位情况。结果:实验所用的水稻株系均为转基因株系;RT-PCR检测发现,在根尖、叶片和幼穗中hpt和OsPIN1a基因均有表达;GUS组织染色结果表明,幼穗、叶片、根系均有GUS活性,其中在幼嫩的组织中表达量最大,如刚萌发种子胚芽鞘、花药和柱头;GUS活性受外源生长素诱导而受生长素极性运输抑制剂(TIBA)的抑制;激光共聚焦显微镜观察发现,OsPIN1a-GFP蛋白主要分布在根尖细胞膜上。结论:实验表明OsPIN1a基因参与了水稻各个器官和组织的发育,这些组织发育可能都受到生长素极性运输的调控。

外文摘要：Objective：In order to explore the function and distribution of auxin efflux carrier protein OsPIN1a in different tissues of rice. Method：The Japonica rice Zhonghua 11 and its over -expressed OsPIN1a gene strains with GFP and GUS reporter gene were chosen as materials. PCR method was used to detect the hygromycin resistance gene marker for transgenetic plants, semi - quantitative RT - PCR was used to detect the expression level of OsPIN1 a in rice root,leaf and panicle, the GUS activity in rice root, leaf and panicle was detected by using GUS staining, and subcellular localization of OsPIN1 a - GFP in the root tip observed by a laser confocal - microscopy~ Result：The results showed that all of transgenic strains were positive by genomic PCR detection; the hygromycin resistance gene marker and OsPIN1 a gene both expressed in rice roots,leaves and panicles by semi - quantitative RT - PCR. By GUS activity staining we found that panicles, leaves and roots all displayed GUS activity. Besides ,the expression of GUS were stronger in young tissues, such as the newly -germinated seeds,coleoptile and stigma. The GUS activity were also induced by IAA and suppressed by polar auxin transport inhibitors （TIBA） . Moreover, the OsPIN1 a - GFP protein mainly located on the cell membrane in rice root tips by a laser confocal - microscopy. Conclusion： The experimental results showed that OsPIN1a gene might involve in the development and morphogenesis of these tissues by polar auxin transport.