文献类型：期刊文献

英文题名：Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors

作者：Wang, Xihong[1,2,3,4];Jin, Lifang[1,2,5];Ji, Shaohui[1,2,4];Guo, Xiangyu[1,2,3,4];Chen, Hongwei[1,2,4];Ji, Weizhi[1,2,4,6]

机构：[1]Chinese Acad Sci, Kunming Primate Res Ctr, Kunming, Yunnan, Peoples R China;[2]Chinese Acad Sci, Kunming Inst Zool, Kunming, Yunnan, Peoples R China;[3]Chinese Acad Sci, Grad Sch, Beijing, Peoples R China;[4]Yunnan Key Lab Anim Reprod Biol, Kunming, Yunnan, Peoples R China;[5]Shaoxing Univ, Coll Life Sci, Shaoxing, Zhejiang, Peoples R China;[6]Kunming Biomed Int, Kunming 650500, Yunnan, Peoples R China

年份：2011

卷号：35

期号：8

起止页码：775

外文期刊名：CELL BIOLOGY INTERNATIONAL

收录：SCI-EXPANDED(收录号：WOS:000293940900003)、、Scopus(收录号：2-s2.0-79959928990)、WOS

基金：This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW-R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Development Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]: and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911].

语种：英文

外文关键词：collagen gel; embryonic stem cell; growth factor; hepatocyte; Rhesus monkey

外文摘要：rES (rhesus monkey embryonic stem) cells have similar characteristics to human ES (embryonic stem) cells, and might be useful as a substitute model for preclinical research. Before their clinical application, it is critical to understand the roles of factors that control the differentiation of ES cells into hepatocytes. Here, we analysed the effect of collagen gels on rES cells differentiation into hepatocytes by stepwise protocols. About 80% of DE (definitive endoderm) cells were generated from rES cells after being treated with activin A. The DE cells were then plated on to collagen gels or type I collagen-coated wells with growth factors to induce hepatocyte differentiation. In type I collagen systems, characteristics of immature hepatocytes were observed, including the expression of immature hepatic genes and the generation of 15 +/- 3% AFP (alpha fetoprotein)/CK (cytokeratin)18 double-positive cells. In collagen gel culture, differentiated cells exhibited typical hepatocyte morphology and expressed adult liver-specific genes. The mRNA expression of AFP (immature hepatic gene) was detected at day 11 but decreased at day 18. In contrast, mRNA expression of albumin (mature hepatic gene) was detected at day 11 and increased at day 18. Compared with type I collagen systems, significantly higher AFP/CK18 double-positive cells (68 +/- 7%) were produced in collagen gel culture. Furthermore, some differentiated cells acquired the hepatocytic function of glycogen storage. However, only immature hepatic genes were observed in collagen gel systems if growth factors were absent. Thus, collagen gels combined with hepatocyte-inducing growth factors efficiently promoted differentiation of hepatocytes from rES.