文献类型：期刊文献

英文题名：Isolation, identification, biological characteristics, and antifungal efficacy of sodium bicarbonate combined with natamycin on Aspergillus niger from Shengzhou nane (Prunus salicina var. taoxingli) fruit

作者：Guo, Tian-Rong[1];Zeng, Qing[1];Yang, Guo[1];Ye, Si-Si[1];Chen, Zi-Yi[1];Xie, Shi-Ying[1];Wang, Hai[1];Mo, Yi-Wei[1]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing, Peoples R China

年份：2023

卷号：13

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000918458900001)、、WOS

基金：This study was supported by the basic public welfare research projects of Zhejiang Province, China (Nos. LGN20C150004 and LGN22C150002).

语种：英文

外文关键词：Prunus salicina var; taoxingli; Aspergillus niger; sodium bicarbonate; natamycin; antifungal mechanism

外文摘要：The fungi causing fruit rot were isolated from symptomatic Shengzhou nane (Prunus salicina var. taoxingli) fruit and were identified as Aspergillus niger by biological characteristics and molecular analysis of the internal transcribed spacer region (rDNA-ITS) and translation elongation factor-1 alpha (TEF-1 alpha) sequences. Optimal growth conditions for A. niger were 30 degrees C, pH 5.0-6.0, and fructose and peptone as carbon and nitrogen sources. The effects of sodium bicarbonate (SBC), natamycin (NT), and combined treatments on A. niger inhibition were investigated. Treatment with 4.0 g/L sodium bicarbonate (SBC) + 5.0 mg/L natamycin (NT) inhibited mycelial growth and spore germination as completely as 12.0 mg/L SBC or 25.0 mg/L NT. SBC and NT treatments disrupted the structural integrity of cell and mitochondria membranes and decreased enzyme activities involved in the tricarboxylic acid (TCA) cycle, mitochondrial membrane potential (MMP), ATP production in mitochondria, and ergosterol content in the plasma membrane, thus leading to the inhibition of A. niger growth. Moreover, experimental results in vivo showed that the rot lesion diameter and decay rate of Shengzhou nane fruit treated with SBC and NT were significantly reduced compared with the control. The results suggest that the combination treatment of SBC and NT could be an alternative to synthetic fungicides for controlling postharvest Shengzhou nane decay caused by A. niger.