文献类型：期刊文献

英文题名：Transmembrane kinase 1-mediated auxin signal regulates membrane-associated clathrin in Arabidopsis roots

作者：Wang, Yutong[1];Yan, Xu[1];Xu, Mei[1];Qi, Weiyang[1];Shi, Chunjie[1];Li, Xiaohong[1];Ma, Jiaqi[1];Tian, Dan[1];Shou, Jianxin[1];Wu, Haijun[1];Pan, Jianwei[1];Li, Bo[1];Wang, Chao[1,2]

机构：[1]Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China

年份：0

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000905857600001)、、Scopus(收录号：2-s2.0-85145369185)、WOS

基金：We dedicate this work in memory of our mentor Prof. Jianwei Pan. His passion for science, kindness, and generosity remain an inspiration to us all. We thank Prof. Jianwei Pan for designing most of the experiments. Moreover, we are thankful to Prof. Tongda Xu and Dr. Yongqiang Yu from the Fujian Agriculture and Forestry University for kindly providing the tmk1-1, tmk3-1, and tmk4-1 mutants. We thank Prof. Ben Scheres, Prof. Sebastian Y. Bednarek, Prof. Jiri Friml, Prof. Rishikesh P. Bhalerao, and Prof. Jian Xu for generously sharing published markers. In addition, we also thank Liang Peng, Liping Guan, Yang Zhao, and Yahu Gao (Core Facility for Life Science Research, Lanzhou University) for technical assistance. This work was supported by grants to Jianwei Pan from the National Natural Science Foundation of China (91754104, 31820103008, and 31670283); grants to Chao Wang from the Youth Program of National Natural Science Foundation of China (31801193) and grants to Haijun Wu from the Startup Foundation for Introducing Talent of Lanzhou University (No. 561120206).

语种：英文

外文关键词：Arabidopsis; auxin; clathrin; endocytosis; PIN2; transmembrane kinase1 (TMK1)

外文摘要：Clathrin-mediated endocytosis (CME) is the major endocytic pathway in eukaryotic cells that directly regulates abundance of plasma membrane proteins. Clathrin triskelia are composed of clathrin heavy chains (CHCs) and light chains (CLCs), and the phytohormone auxin differentially regulates membrane-associated CLCs and CHCs, modulating the endocytosis and therefore the distribution of auxin efflux transporter PIN-FORMED2 (PIN2). However, the molecular mechanisms by which auxin regulates clathrin are still poorly understood. Transmembrane kinase (TMKs) family proteins are considered to contribute to auxin signaling and plant development; it remains unclear whether they are involved in PIN transport by CME. We assessed TMKs involvement in the regulation of clathrin by auxin, using genetic, pharmacological, and cytological approaches including live-cell imaging and immunofluorescence. In tmk1 mutant seedlings, auxin failed to rapidly regulate abundance of both CHC and CLC and to inhibit PIN2 endocytosis, leading to an impaired asymmetric distribution of PIN2 and therefore auxin. Furthermore, TMK3 and TMK4 were shown not to be involved in regulation of clathrin by auxin. In summary, TMK1 is essential for auxin-regulated clathrin recruitment and CME. TMK1 therefore plays a critical role in the establishment of an asymmetric distribution of PIN2 and an auxin gradient during root gravitropism.