文献类型：期刊文献

英文题名：Identification and functional characterization of a human sTRAIL homolog, CasTRAIL, in an invertebrate oyster Crassostrea ariakensis

作者：Yang, Shoubao[1,2];Wu, Xinzhong[1]

机构：[1]Zhejiang Univ, Coll Anim Sci, Lab Marine Life Sci & Technol, Hangzhou 310029, Zhejiang, Peoples R China;[2]Shaoxing Univ, Shaoxing, Zhejiang, Peoples R China

年份：2010

卷号：34

期号：5

起止页码：538

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000275511900008)、、WOS

基金：This work was supported by research grant from the National Natural Science Foundation of China (30671619) and the Key Scientific Program of Zhejiang Province (2004C23041). We thank Dr. Patrick C. Hanington, University of New Mexico, Albuquerque, NM 87131, USA, for critical review of the manuscript.

语种：英文

外文关键词：Oyster Crassostrea ariakensis; Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); CasTRAIL; Anti-RLO immune

外文摘要：Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) is one of the tumor necrosis factor (TNF) superfamily members, participating in many biological processes including apoptosis and immune responses. In present study, a novel human soluble TRAIL (sTRAIL) homolog, CasTRAIL was identified from the oyster, Crassostrea ariakensis. CasTRAIL has a 99% and 98% similarity to human sTRAIL over the cDNA sequence and the amino acid sequence, respectively. It mostly distributes in tissues of the oyster defense system and was mainly localized at cell membrane, and has no cytotoxicity to normal hemocytes of oyster. The phosphorylation state of MAP kinases revealed that CasTRAIL induced a rapid increase in the phospho-ERK and phospho-p38 levels. which indicated that the MAN pathway was involved in CasTRAIL-mediated signaling. In addition, CasTRAIL also showed an ability of anti-RLO infection which might be through the p38-MAPK activation pathway. Present studies provide an understanding and insight of the biological functions of CasTRAIL. (C) 2010 Elsevier Ltd. All rights reserved.