文献类型：期刊文献

中文题名：细胞周期素D1和p27^(kip1)在肾癌中的表达及其意义

英文题名：Expression of cyclin D1 and p27^(kip1) in renal cell carcinoma and its significance

作者：葛建荣[1];任国平[2];姚航平[2]

机构：[1]绍兴文理学院医学院病理学教研室;[2]浙江大学医学院附属第一医院

年份：2007

卷号：36

期号：5

起止页码：483

中文期刊名：浙江大学学报：医学版

收录：MEDLINE(收录号：17924468)、CSTPCD、、Scopus(收录号：2-s2.0-39049149185)、CSCD_E2011_2012、CSCD、PubMed

基金：浙江省卫生厅基金资助项目(2000B091)

语种：中文

中文关键词：癌;肾细胞/病理学;肾肿瘤/病理学;细胞周期蛋白质类/生物合成;细胞周期蛋白D1/生物合成;肿瘤分期;基因;肿瘤抑制;免疫组织化学;存活率

外文关键词：Carcinoma, renal cell/pathol; Kidney neoplasms/pathol; Cell cycle proteins/ biosyn; Cyclin D1/biosyn; Neoplasm staging; Genes, tumor suppressor; Immunohistochemistry; Survival rate

中文摘要：目的:探讨cyclin D1、p27kip1在普通型肾细胞癌(conventional renal cell carcinoma,CRCC)发生、发展中的作用。方法:用定量RT-PCR和Western-blot方法检测25例CRCC组织和10例肿瘤远端的正常肾皮质中cyclin D1和p27kip1的mRNA和蛋白的含量;用免疫组化方法检测76例CRCC中cyclin D1、p27kip1的表达;分析cyclin D1、p27kip1的表达与肿瘤分期、分级及术后生存率的相关性。结果:CRCC中cyclin D1的mRNA和蛋白含量分别为0.488±0.399和1.069±0.371,高于正常对照组0.089±0.066和0.281±0.253(P均<0.01);cyclin D1的表达与肿瘤体积大小有关,体积大者cyclin D1高表达(P<0.05);CRCC中p27kip1mRNA和蛋白含量分别为0.191±0.111和0.146±0.051,低于正常对照组0.374±0.216和1.004±0.318(P均<0.01),随着p27kip1表达的降低,肿瘤的细胞核Fuhrman分级、TNM分期增高;p27kip1阳性组患者的5年生存率为84.26%,高于p27kip1阴性组25.25%(P<0.01)。结论:cyclin D1高表达和p27kip1的低表达与CRCC的发生有关;p27kip1的低表达可能促进肿瘤的演进,检测p27kip1可作为评价CRCC预后的参考指标。

外文摘要：Objective： To investigate the role of cyclin D1 and p27^kip1 in the occurrence and development of conventional renal cell carcinoma（CRCC）. Methods： RT-PCR and Western-blot were used to detect mRNA and protein contents of cyclin D1 and p27^kip1 in 25 CRCCs and 10 normal renal tissue distant to tumor. Immunohistochemistry was used to investigate the expression of cyclin D1 and p27^kip1 in pathological tissue sections of 76 CRCCs. The relationship between those index and clinicopathological parameters was analyzed statistically. Results： In CRCC,the expression of cyclin D1 was higher than that of the control group. The higher cyclin D1 content was related to big tumor size （P〈0.05） ; The expression of p27^kip1 was lower than that of the control group,and the lower p27^kip1 was related to higher nuclear grade and TNM stage（P〈0.01）. The 5-year cumulative survival rate of the p27^kip1 high expression group is longer than that of the low group（P〈0. 01）. Conclusions. The excessive expression of cyclin D1 and lower expression of p27^kip1 play an important role in the carcinogenesis of CRCC. The lower expression Of p27^kip1 may affect the progression of the tumors. The detection of p27^kip1 may be as a reference marker in the prognosis of CRCC.