文献类型：期刊文献

中文题名：NO诱导IAA和O_(2)·^(-)积累于侧根尖端促进水稻侧根生长

英文题名：Nitric oxide induced accumulation of IAA and O_(2)·^(-) in lateral root was beneficial to lateral root development in rice seedling

作者：朱雄梅[1];蔡慧楠[2];刘瑞琪[2];林佩锦[2];郞双怡[2];黄悦[2];邓舒琪[1];莫亿伟[2];金晨钟[1]

机构：[1]湖南人文科技学院农田杂草防控技术与应用协同创新中心,湖南娄底417000;[2]绍兴文理学院生命科学学院,浙江绍兴312000

年份：2022

卷号：43

期号：3

起止页码：513

中文期刊名：农业现代化研究

外文期刊名：Research of Agricultural Modernization

收录：CSTPCD、、国家哲学社会科学学术期刊数据库、北大核心、CSCD、北大核心2020、CSCD_E2021_2022

基金：浙江省科技厅公益项目(LGN19C130003);湖南省科技创新计划项目(2019NK4170);湖南省教育厅项目(20C1031)。

语种：中文

中文关键词：水稻;侧根;一氧化氮;硝普纳;生长素;超氧阴离子

外文关键词：rice;lateral root;nitric oxide;sodium nitroprusside;auxin;superoxide anion

中文摘要：为了探讨NO和超氧阴离子对水稻种子根侧根生长的影响,以DR5-GUS标记IAA的转基因水稻为材料,种子催芽萌发后,用含有不同浓度的NO供体、NO合成酶抑制剂和清除剂溶液来培养萌发3 d的水稻幼苗,在培养的第3 d和第5 d分别测量初生根长度和初生根上侧根数量,并通过DAF-2DA对NO、GUS对IAA和NBT对超氧阴离子(O_(2)·^(-))染色后,在显微镜下观察NO、IAA和O_(2)·^(-)在侧根的定位,分析与NO合成相关的基因表达。结果表明,NO和IAA主要分布在根中柱的维管束部分以及侧根起始形成处和侧根根尖,O_(2)·^(-)只极性分布在侧根起始部位和侧根根尖。当NO合成受到抑制或清除时,NO、IAA和O_(2)·^(-)在侧根的积累量明显减少,侧根数量及初生根长度也均显著下降。荧光定量PCR分析发现,外源的NO供体SNP处理均能显著诱导水稻OsNIA1和OsNIA2基因表达,而NO合成酶抑制剂处理则抑制OsNIA1和OsNIA2基因表达。基于我们的实验结果,NO作为一种重要的信号物质参与初生根和侧根的生长,并需IAA和O_(2)·^(-)极性积累于侧根的起始部位,从而达到促进侧根的形成,当NO合成受阻时初生根和侧根生长均受到抑制。

外文摘要：To explore the effects of nitric oxides(NO)and superoxide anion on the lateral root development in rice seedling,DR5-GUS-labeled transgenic rice seedling after germination for three days was used as materials and was cultured with the solutions of different concentrations of NO donor,NO synthetase inhibitor,and NO scavenger,respectively.The lateral root growth in the primary root and the number of lateral roots growth were measured after being cultured in 3 days and 5 days.The detection of NO was performed with 4,5-diamino-fluorescein diacetate(DAF-2DA)the detection of IAA histochemical location was performed withβ-glucuronidase(GUS)activity,and the detection of superoxide anion O_(2)·^(-) histochemical location was performed with nitro blue tetrazoliums(NBT)to analyze the expressions of genes relative to the NO synthesis.The results showed that NO and IAA were mainly distributed in the vascular bundle,the lateral root of the beginning part,and the root tip of lateral root,whereas O_(2)·^(-)was a polar distribution in the lateral root of the beginning part and the root tip of lateral root.When NO synthesis was inhibited or superoxide radicals were scavenged,the accumulation of NO,IAA,and O_(2)·^(-)was significantly reduced in the lateral root,and the number of lateral root and length of primary root was significantly decreased.Real-time PCR analysis found that treating with the exogenous NO donor SNP could significantly induce the expressions of OsNIA1 and OsNIA2 gene,whereas the treatment with NO the synthetase inhibitor could inhibit the expressions of OsNIA1 and OsNIA2.Based on these results,it was speculated that NO might be used as an important signal substance to participate in the growth of the primary root and lateral roots,and IAA and O_(2)·^(-)were required to accumulate in the parts of lateral root of the beginning part and root tip of lateral root to promote the lateral root formation.When NO synthesis was blocked,the primary root and lateral root growth were inhibited.