文献类型：期刊文献

英文题名：Role of ultrasound and microbubble-mediated heat shock protein 72 siRNA on ischemia-reperfusion liver injury in rats

作者：Yan, Chungen[1];Zhu, Dongfang[2];Huang, Danwen[1];Xia, Guoyuan[3]

机构：[1]Shaoxing Univ, Affiliated Hosp, Dept Gastroenterol, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Affiliated Hosp, Dept Lab Med, Shaoxing 312000, Zhejiang, Peoples R China;[3]Shaoxing Univ, Affiliated Hosp, Dept Ultrasound Diag, Shaoxing 312000, Zhejiang, Peoples R China

年份：2015

卷号：8

期号：4

起止页码：5746

外文期刊名：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE

收录：SCI-EXPANDED(收录号：WOS:000358377700112)、、Scopus(收录号：2-s2.0-84935878408)、WOS

基金：The study was supported by Zhejiang Provincial Natural Science Foundation of China (No. Y2100961, LY15H180008) and Shaoxing Science and Technology Project (2015014001).

语种：英文

外文关键词：Heat shock protein 72; siRNA; gene transfection; ultrasound micro-bubble contrast agents; ischemia-reperfusion liver injury

外文摘要：Objective: To explore the ultrasound-guided gene transfection as well as the role of heat shock protein 72 (HSP72) siRNA combined with ultrasound micro-bubble contrast agents on rat hepatic ischemia-reperfusion injury. Methods: 72 SD rats were divided into non-surgery group (group N), sham-operation group (group P) and liver ischemia-reperfusion groups (I/R). In each group, rats were further divided into 4 subgroups according to the different intravenous treatment: 220 ul saline solution (group A); 20 ul HSP72 siRNA plasmid vector + 200 ul saline solution (group B); 20 ul HSP72 siRNA plasmid vector + 200 ul ultrasound microbubble contrast agent (group C); 20 ul HSP72 siRNA plasmid vector + 200 ul ultrasound microbubble contrast agent + ultrasonic irradiation target region with MI1.0 (group D). Results: Certain degree hepatic tissue injury was observed in rats of group I/R A, B and C. The expressions of liver tissue HSP72 mRNA and HSP72 protein and the concentrations of peripheral blood HSP72, ALT and TNF-alpha were significantly increased at each I/R subgroup (vs group N and group P, P < 0.01). Among them, the plasma concentrations of ALT, HSP72, and TNF-a and the liver tissue expressions of HSP72 mRNA and HSP72 protein at group A were significantly higher than groups B, C and D (P < 0.01). And group D was significantly lower than that of group A, B and C (P < 0.01). Conclusion: The liver tissue expressions of HSP72 mRNA and HSP72 protein and the liver injury degree of ischemia-perfusion were significantly reduced after the HSP72 siRNA was combined with micro-bubble and radiated directionally by ultrasound.