文献类型：期刊文献

英文题名：miRNA-331-3p Affects the Proliferation, Metastasis, and Invasion of Osteosarcoma through SOCS1/JAK2/STAT3

作者：Zu, Dan[1];Dong, Qi[2];Chen, Sunfang[2];Chen, Yongde[1];Yao, Jun[2];Zou, Yubin[2];Lin, Jiawei[2];Fang, Bin[2];Wu, Bing[2]

机构：[1]Shaoxing Univ, Cent Hosp, Cent Lab, Shaoxing 312030, Peoples R China;[2]Shaoxing Univ, Cent Hosp, Dept Spine Surg, Shaoxing 312030, Peoples R China

年份：2022

卷号：2022

外文期刊名：JOURNAL OF ONCOLOGY

收录：SCI-EXPANDED(收录号：WOS:000868908500011)、、Scopus(收录号：2-s2.0-85139727173)、WOS

基金：The authors would like to thank the teachers in Zhejiang Provincial Laboratory Animal Center for their kindly help in mastering the skills of animal experiments. In addition, the authors thank the teachers in the Sir Run Run Shaw Hospital for their guidance in the Experiment. The preprint source is https://www.researchsquare.com/article/rs-1190352/v1. This work was supported by the Medical and Health Science and Technology Plan Project of Zhejiang Province (grant number 2020KY990).

语种：英文

外文摘要：MicroRNAs (miRNAs) are regulatory small noncoding RNAs that play a key role in several types of cancer. It has been reported that miR-331-3p is involved in the development and progression of various cancers, but there are few reports regarding osteosarcoma (OS). The public GEO database was used to analyze the survival difference of miR-331-3p in OS organizations. The level of cell proliferation assay was assessed by CCK-8 and colony formation. First, transwell and wound-healing assays were used to detect the transfer and invasion ability of miR-331-3p in OS. Second, TargetScan, miRDBmiR, TarBase, and dual-luciferase reporter gene assays were used to determine SOCS1 as a targeted regulator. Third, Western blot and immunohistochemistry were used to detect the expression of protein levels. Finally, a mouse model of subcutaneously transplantable tumors is used to evaluate the proliferation of OS in vivo. The low expression of miR-331-3p was negatively correlated with the overall survival of OS patients. Overexpression of miR-331-3p significantly inhibited cell proliferation, metastasis, and invasion. Moreover, miR-331-3p affected the occurrence and development of osteosarcoma by targeting the SOCS1/JAK2/STAT3 signaling pathway. Therefore, miR-331-3p reduces the expression of SOCS1 by combining with its 3 ' UTR, thereby activating the JAK2/STAT3 signaling pathway to regulate the progression of OS. This provides a new theoretical basis for the treatment of osteosarcoma.