文献类型：期刊文献

英文题名：Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems

作者：Jin, Li-Fang[1,2,3];Li, Jin-Song[2,3]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci,Inst Biochem & Ce, Shanghai 200031, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China

年份：2016

卷号：37

期号：4

起止页码：205

外文期刊名：ZOOLOGICAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000383250900003)、、Scopus(收录号：2-s2.0-85016299082)、WOS

语种：英文

外文关键词：CRISPR/Cas9; Genetic modification; Haploid embryonic stem cells; Mouse

外文摘要：With the development of high-throughput sequencing technology in the post-genomic era, researchers have concentrated their efforts on elucidating the relationships between genes and their corresponding functions. Recently, important progress has been achieved in the generation of genetically modified mice based on CRISPR/Cas9 and haploid embryonic stem cell (haESC) approaches, which provide new platforms for gene function analysis, human disease modeling, and gene therapy. Here, we review the CRISPR/Cas9 and haESC technology for the generation of genetically modified mice and discuss the key challenges in the application of these approaches.