文献类型：期刊文献

英文题名：Abscisic acid enhances DNA damage response through the nuclear shuttling of clathrin light chain 2 in plant cells

作者：Jiang, Jieming[1];Zhu, Qiuhua[2];Li, Yang[1];Wu, Jiayuan[1];Zou, Chenghuan[1];Li, Hongbo[1];Lei, Xiaohui[1];Zhang, Fangyuan[1];Lin, Yujie[1];Cao, Rui[1];Cao, Ying[1];Mo, Jiawang[1];Lai, Jianbin[1];Zhao, Jiangzhe[3];Wang, Chao[2];Yang, Chengwei[1]

机构：[1]South China Normal Univ, Sch Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China;[3]Zhejiang Normal Univ, Coll Life Sci, Jinhua 321004, Peoples R China

年份：2025

卷号：11

期号：24

外文期刊名：SCIENCE ADVANCES

收录：SCI-EXPANDED(收录号：WOS:001508114400031)、、EI(收录号：20252518646204)、Scopus(收录号：2-s2.0-105008436128)、WOS

基金：The project was supported by grants from the Major Program of Guangdong Basic and Applied Research (2019B030302006), the National Natural Science Foundation of China (32370775 and 32270292), and the Program for Changjiang Scholars, Guangdong Provincial Pearl River Talent Plan (2019QN01N108).

语种：英文

外文关键词：Cells - Chains - Damage tolerance - Plant cell culture - Plant shutdowns - Plants (botany) - Samarium compounds

外文摘要：DNA damage arises from various environmental stresses, and ABA is well known for its roles in plant stress resistance. However, its function in plant DNA damage tolerance remains unclear. In this study, we showed that ABA supplementation significantly enhances plant tolerance to DNA-damaging treatments. SnRK2.2 and SnRK2.3 kinases in the ABA signaling pathway are pivotal in this process. These kinases interact with clathrin light chain 2 (CLC2), facilitating its phosphorylation and nuclear translocation in response to Zeocin and ABA treatment. In the nucleus, CLC2 interacts with ADA2b, an adaptor protein crucial for recruiting SMC5/6 complex to the double-strand break (DSB) sites. The enhanced nuclear localization of CLC2 is essential for the accurate localization of ADA2b at DSB sites. Collectively, our study uncovers that ABA enhances plant DNA damage tolerance with a distinct function of CLC2 in genomic stability maintaining, thereby improving our understanding of DNA damage tolerance mechanisms in plants.