文献类型：期刊文献

中文题名：增殖性细胞核抗原和c-Fos蛋白参与调控人胚胎脊髓的发育

英文题名：Proliferating cell nuclear antigen and c-Fos protein regulating development of the human embryonic spinal cord

作者：洪雅丹[1];张泳[1];刘学红[1]

机构：[1]绍兴文理学院医学院组织学与胚胎学教研室

年份：2011

卷号：42

期号：6

起止页码：836

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD、、Scopus、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：绍兴市科技计划资助项目(2009A33025);浙江省新苗人才计划资助项目;绍兴市大学生科技创新资助项目

语种：中文

中文关键词：脊髓;增殖性细胞核抗原;c—Fos蛋白;免疫组织化学;人胚胎

外文关键词：Spinal cord; Proliferating cell nuclear antigen; c-Fos protein; Immunohistochemistry; Human embryo

中文摘要：目的探讨人胚胎发育早、中期脊髓内神经细胞增殖与凋亡的变化规律及其相关蛋白增殖性细胞核抗原(PCNA)和c-Fos在脊髓中的分布规律及其表达意义。方法应用免疫组织化学法检测第2～4月龄段人胚胎(共16例)脊髓组织中央管、前角和后角处PCNA和c-Fos蛋白的表达情况。结果第2个月龄段,人胚脊髓基板处无PCNA蛋白阳性表达,中央管神经上皮细胞和翼板均有PCNA蛋白阳性表达;而c-Fos蛋白在脊髓基板、翼板和中央管神经上皮细胞处均呈阳性表达。随着胎龄的增大,PCNA蛋白在脊髓前角、中央管和后角均有阳性表达;c-Fos蛋白在脊髓前角神经细胞的阳性表达数量和染色强度均值均呈先降低再升高的趋势(P<0.01),而在脊髓后角的阳性表达无明显变化。结论 PCNA和c-Fos蛋白参与调控人胚胎神经管神经上皮细胞的生长发育。

外文摘要：Objective To explore effects of proliferating cell nuclear antigen （PCNA）and c-Fos protein on the early and middle embryonic development of the human spinal cord. Methods Using immunohistochemical method, the expression of PCNA and c-Fos were investigated in the central canal, anterior horn and posterior horn of spinal cord of 16 human embryos aged at the second to fourth month of gestation. Results At the second month of gestation, there were the positive PCNA immunohistochemical reaction in the central canal and alar plate of the spinal cord, but not in the basal plate. The c-Fos immunohistochemical positive reaction were localized in the epithelial cells of the central canal, alar plate and basal plate of the spinal cord. Following growth, the positive immunohistochemieal staining for PCNA was also found in the epithelial cells of the central canal, anterior horn, and posterior horn of the spinal cord. The average intensity and cell number of the e-Fos immunohistochemical positive profiles decreased first, and then increased in spinal cord anterior horn （P 〈 0. 01） , but no changes was found in the posterior horn of the spinal cord. Conclusion PCNA and c-Fos proteins may regulate the growth and development of the neuroepithelial cells of the neural tube of the human embryo.