文献类型：期刊文献

英文题名：Identification of specific antigenic epitope at N-terminal segment of enterovirus 71 (EV-71) VP1 protein and characterization of its use in recombinant form for early diagnosis of EV-71 infection

作者：Zhang, Jianhua[1,2];Jiang, Bingfu[1];Xu, Mingjie[1];Dai, Xing[3];Purdy, Michael A.[4];Meng, Jihong[1]

机构：[1]Southeast Univ, Sch Med, Dept Microbiol & Immunol, Nanjing, Jiangsu, Peoples R China;[2]Shaoxing Univ, Sch Med, Dept Microbiol & Immunol, Shaoxing, Zhejiang, Peoples R China;[3]Southeast Univ, Affiliated Zhongda Hosp, Sch Med, Dept Dermatol, Nanjing, Jiangsu, Peoples R China;[4]Ctr Dis Control & Prevent, Div Viral Hepatitis, Atlanta, GA 30333 USA

年份：2014

卷号：189

起止页码：248

外文期刊名：VIRUS RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000341557700033)、、Scopus(收录号：2-s2.0-84905981595)、WOS

基金：We are deeply indebted to Dr. Xianhui You for providing human clinical specimens collected from HFMD patients. We are grateful to Min Dong, Dr. Chen Dong and Dr. Jiuhong Liang for their valuable technical assistance and suggestions. We thank Dr. Chong-Gee Teo for a critical reading of the manuscript. Disclaimer : This information is distributed solely for the purpose of pre-dissemination peer review under applicable information quality guidelines. It has not been formally disseminated by the Centers for Disease Control and Prevention/Agency for Toxic Substances and Disease Registry. It does not represent and should not be construed to represent any agency determination or policy. Use of trade names is for identification only and does not imply endorsement by the U.S. Department of Health and Human Services, the Public Health Service, or the Centers for Disease Control and Prevention. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

语种：英文

外文关键词：Enterovirus 71; Hand, Foot and mouth disease; Viral capsid protein; Early diagnosis

外文摘要：Human enterovirus 71 (EV-71) is the main etiologic agent of hand, foot and mouth disease (HFMD). We sought to identify EV-71 specific antigens and develop serologic assays for acute-phase EV-71 infection. A series of truncated proteins within the N-terminal 100 amino acids (aa) of EV-71 VP1 was expressed in Escherichia coli. Western blot (WB) analysis showed that positions around 11-21 aa contain EV-71-specific antigenic sites, whereas positions 1-5 and 51-100 contain epitopes shared with human coxsackievirus A16 (CV-A16) and human echovirus 6 (E-6). The N-terminal truncated protein of VP1, VP1(6-43), exhibited good stability and was recognized by anti-EV-71 specific rabbit sera. Alignment analysis showed that VP1(6-43) is highly conserved among EV-71 strains from different genotypes but was heterologous among other enteroviruses. When the GST-VP1(6-43) fusion protein was incorporated as antibody-capture agent in a WB assay and an ELISA for detecting anti-EV-71 IgM in human sera, sensitivities of 91.7% and 77.8% were achieved, respectively, with 100% specificity for both. The characterized EV-71 VP1 protein truncated to positions 6-43 aa has potential as an antigen for detection of anti-EV-71 IgM for early diagnosis of EV-71 infection in a WB format. (C) 2014 Elsevier B.V. All rights reserved.