文献类型：期刊文献

英文题名：Hic12, a novel acidic matrix protein promotes the transformation of calcite into vaterite in Hyriopsis cumingii

作者：Luo, Wen[1];Jiang, Rui[1];Ren, Gang[1];Jin, Can[1]

机构：[1]Shaoxing Univ, Sch Life Sci, Huancheng West Rd 508, Shaoxing 312000, Peoples R China

年份：2022

卷号：261

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000804968000001)、、WOS

基金：This work was supported by the Scitech Special Commissioners Project of Zhejiang Province-Pearl Quality Improvement and Industrialization (2020-2024) . We also thank Dr. Zhong CHEN and Yuan CHENG from the Instrumentation and Service Center for Molecular Sciences at Westlake University for assistance with the Raman spectroscopy measurements and data analysis. We also thank International Science Editing (http:// www.internationalscienceediting.com ) for editing this manuscript.

语种：英文

外文关键词：Matrix protein; Pearl; Vaterite; Nacreous layer; Biomineralization; Hyriopsis cumingii

外文摘要：Shell acidic matrix proteins are widely considered to be essential for shell formation given their low affinity and high loading for calcium ion. In the present study, a novel matrix protein, hic12, was isolated from the mantle of Hyriopsis cumingii. High expression in tissue and positive signals with in situ hybridization were detected in the mantle center and mantle pallium, indicating that hic12 mainly participated in the biomineralization of the shell nacreous layer. The expression pattern of hic12 in the pearl sac during early pearl formation indicated that it was involved in pearl biomineralization. Moreover, the recombinant protein, rGST-Hic12, was successfully expressed and purified. The addition of rGST-Hic12 could accelerate the calcium carbonate deposition rate, change the morphology of crystals, and promote the conversion of calcite to vaterite. These results may provide new insights into the molecular mechanisms of aragonite mollusk shell formation.