文献类型：期刊文献

英文题名：Characterization of MSTN/GDF11 gene from shrimp Macrobrachium nipponense and its expression profiles during molt cycle and after eyestalk ablation

作者：Shen, Wenying[1];Ren, Gang[1];Zhu, Yaorong[1];Zhang, Xiaodong[1]

机构：[1]Shaoxing Univ, Dept Biol, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China

年份：2015

卷号：37

期号：5

起止页码：441

外文期刊名：GENES & GENOMICS

收录：SCI-EXPANDED(收录号：WOS:000353029500003)、、WOS

基金：This study was supported by the Public Project of Zhejiang Province, P. R. China (No. 2013C32068) and the Foundation of Zhejiang Education Committee of China (Y201225243).

语种：英文

外文关键词：MSTN/GDF11; Macrobrachium nipponense; Muscle growth; Development stages; Molting; Eyestalk ablation

外文摘要：Myostatin (MSTN), also known as the growth differentiation factor-8 (GDF-8), belongs to the transforming growth factor-beta superfamily. MSTN is a negative regulator of muscle development in vertebrates. However, the transcriptional regulation of MSTN in freshwater crustaceans is still unclear. In this study, a cDNA encoding for MSTN/GDF11 (Mn-MSTN/GDF11) was cloned from the oriental freshwater shrimp, Macrobrachium nipponense. The full-length cDNA sequence of Mn-MSTN/GDF11 was composed of 1733 nucleotides, including a 5' UTR of 119 nucleotides, an open read frame of 1359 nucleotides, and a 3' UTR of 255 nucleotides. The predicted peptide of Mn-MSTN/GDF11 has 452 amino acids with the conserved RXXR cleavage site and nine cysteines. Tissue-specific expression pattern showed that Mn-MSTN/GDF11 is mainly expressed in abdominal muscle. During the course of embryonic development, expression level of Mn-MSTN/GDF11 could be detected after gastrul stage, and reached at the highest level at embryonized-zoea stage. During molt cycle, expression level of Mn-MSTN/GDF11 mRNA was up-regulated significantly at early postmoult stage, but down-regulated gradually in the following molt stages. In the period of 14 days after eyestalk ablation, Mn-MSTN/GDF11 transcripts were significantly decreased in abdominal muscle and heart, but increased in thoracic muscle. The results of this study indicated that Mn-MSTN/GDF11 may play a role in molt cycle and be regulated by hormone secreted in eyestalk.