文献类型：期刊文献

英文题名：A Rare Strain Actinomadura geliboluensis Was First Isolated from the Bronchoalveolar Lavage Fluid of a Patient with Pneumonia

作者：Yu, Yefu[1];Yang, Guier[2];Wang, Yanan[1];Jin, Faxiang[1];Wang, Huiyu[1];Yu, Zhongqiang[3];Li, Lanqing[4];Li, Xiangcheng[4];Gao, Junshun[4];Xu, Wenfang[1]

机构：[1]Shaoxing Univ, Affiliated Hosp, Dept Clin Lab, 999 Zhongxing South Rd, Shaoxing 312000, Peoples R China;[2]Shaoxing Univ, Emergency Ward Affiliated Hosp, Shaoxing 312000, Peoples R China;[3]Shaoxing Univ, Affiliated Hosp, Dept Imaging, Shaoxing 312000, Peoples R China;[4]Key Lab Precis Med Diag & Monitoring Res Zhejiang, 3 Qingchun East Rd, Hangzhou 310016, Peoples R China

年份：2023

卷号：16

起止页码：3101

外文期刊名：INFECTION AND DRUG RESISTANCE

收录：SCI-EXPANDED(收录号：WOS:000994618300001)、、Scopus(收录号：2-s2.0-85162683245)、WOS

基金：Funding This study was supported by the Medical Science and Health Science Program of Zhejiang Province (2020KY334) .

语种：英文

外文关键词：Actinomadura geliboluensis; pulmonary actinomycosis; antibiotic resistance; next generation sequencing; genomics

外文摘要：Background: Actinomadura geliboluensis was first isolated in 2012 in Gelibolu, Canakkale, Turkey, and has not been reported to be isolated from humans until now. We have isolated it from the bronchoalveolar lavage fluid (BLF) of a patient with pneumonia and found its drug resistance. It is the first time that Actinomadura geliboluensis has been isolated from humans since its discovery and naming. This case may provide new ideas and methods for the clinical diagnosis and treatment of pulmonary actinomycosis.Case Description: The patient was a 75-year-old male who was hospitalized in a township hospital and failed to improve after penicillin treatment. After admission to our hospital, the patient was treated with piperacillin/tazobactam according to clinical guidelines for 14 days. Actinomadura geliboluensis was isolated from the patient's BLF and was identified by 16S rRNA sequencing. This report shows the biological characteristics and in vitro drug susceptibility testing, as well as the genomics analysis based on next-generation sequencing (NGS). The results demonstrated that Actinomadura geliboluensis was easy to be mistakenly identified as Actinomyces dental caries by using the Merieux ANC identification card. Based on the MIC test, Actinomadura geliboluensis was susceptible to tetracyclines, quinolones and sulfonamides, but resistant to carbapenems, penicillins and cephalosporins. The K-B test results showed Actinomadura geliboluensis was highly sensitive to piperacillin/tazobactam. Genomic analysis based on NGS showed that the Actinomadura geliboluensis belongs to Planobispora rosea EF-Tu mutants conferring resistance to inhibitor GE2270A, AAC (3)-VIIa, vanRO, chrB, and mexY.Conclusion: Actinomycetes is generally sensitive to Penicillin but Actinomadura geliboluensis is not. In vitro drug susceptibility test is needed to support individualized drug use to avoid delay in the disease.