文献类型：期刊文献

英文题名：Surface modification with BSA blocking based on in situ synthesized gold nanoparticles in poly(dimethylsiloxane) microchip

作者：Fan, Da-He[2];Yuan, Shi-Wei[2];Shen, Yong-Miao[1]

机构：[1]Shaoxing Univ, Dept Chem, Shaoxing 312000, Peoples R China;[2]Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224003, Peoples R China

年份：2010

卷号：75

期号：2

起止页码：608

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：SCI-EXPANDED(收录号：WOS:000276921900032)、、EI(收录号：20094912521172)、Scopus(收录号：2-s2.0-70549106850)、WOS

基金：The work was supported by Zhejiang Provincial Natural Science Foundation (Y4080395) and the Applied Chemistry Construction Foundation of Yancheng Institute of Technology (XKY2009016).

语种：英文

外文关键词：Microchip capillary electrophoresis; PDMS-gold nanoparticles composite films; Surface modification

外文摘要：A stable BSA blocking poly(dimethylsiloxane) (PDMS) microchannel was prepared based on in situ synthesized PDMS-gold nanoparticles composite films. The modified microchip could successfully suppress protein adsorption. The assembly was followed by contact angle, charge-coupled device (CCD) imaging, electroosmotic flow (EOF) measurements and electrophoretic separation methods. Contact angle measurements revealed the coated surface was hydrophilic, water contact angle for coated chips was 45.2 degrees compared to a water contact angle for native PDMS chips of 88.5 degrees. The coated microchips exhibited reproducible and stable EOF behavior. With FITC-labeled myoglobin incubation in the coated channel, no fluorescence was observed with CCD image, and the protein exhibited good electrophoretic effect in the modified microchip. (C) 2009 Elsevier B.V. All rights reserved.