文献类型：期刊文献

英文题名：iTRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams

作者：Yu, Bin[1,2];Zhu, Hong-Dan[1,2];Shi, Xiao-Liang[1,2];Chen, Pan-Pan[2];Sun, Xiang-Mei[2];Xia, Gui-Yu[1,2];Fang, Min[1,2];Zhong, Yong-Xing[1,2];Tang, Xiao-Li[1,2];Zhang, Tao[1,2];Pan, Hai-Tao[1,2]

机构：[1]Shaoxing Matern & Child Hlth Care Hosp, Shaoxing 312000, Peoples R China;[2]Shaoxing Univ, Obstet & Gynecol Hosp, Shaoxing, Peoples R China

年份：2021

卷号：18

期号：1

外文期刊名：CLINICAL PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:000687146300001)、、Scopus(收录号：2-s2.0-85113219678)、WOS

基金：This work was supported by the National Natural Science Foundation of China (82071729), the Science Technology Department of Zhejiang Province, China (LGF21H040004, LGF19H040004, LGD20H040001, 2018C37102) and the Health Commission of Zhejiang Province, China (2021KY375, 2019RC296, 2019KY717, 2018KY847); the Science Technology Department of Shaoxing, China (2020A13034, 2020A13035).

语种：英文

外文关键词：Preeclampsia; Offspring; iTRAQ; Gamete and Embryo-Fetal Origins of Adult Diseases; Thoracic aortic

外文摘要：Background: Preeclampsia and gestational hypertension can cause vascular function impairment in offspring. In our previous work, we described the protein expression profiles of umbilical artery tissues from patients with preeclampsia. Methods: To gain insights into the mechanisms of vascular dysfunction in adult rats born to preeclamptic dams, we analyzed thoracic aorta tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS. Results: By using the iTRAQ method, we analyzed 1825 proteins, of which 106 showed significantly different expression in the thoracic aortic. Ingenuity pathway analysis (IPA) showed that the majority of differentially expressed proteins (DEPs) were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, had the strongest effect on cardiovascular function. The expression of G6PD was upregulated in thoracic aorta tissues, as confirmed by Western blotting. The expression of two other vascular function-related proteins, cysteine- and glycine-rich protein 2 (CSRP2) and tubulin alpha-4 A (TUBA4A), was upregulated, as demonstrated by mass spectrometry (MS). Conclusions: Although the results require further functional validation, these data provide novel findings related to vascular function impairment in the adult offspring of preeclamptic mothers.