文献类型：期刊文献

英文题名：Worenine enhances the sensitivity of non-small cell lung cancer A549/DDP cells to cisplatin via HIF-1α/BNIP3-mediated mitophagy

作者：Yang, Guobiao[1];Xu, Mengmin[1];Wu, Mingliang[1];Zhang, Ya[1];Yang, Feiyan[1]

机构：[1]Shaoxing Univ, Affiliated Hosp, Shaoxing Municipal Hosp, Dept Resp & Crit Care Med, 999 Zhongxing South Rd, Shaoxing 312000, Peoples R China

年份：2025

卷号：44

期号：4

起止页码：317

外文期刊名：GENERAL PHYSIOLOGY AND BIOPHYSICS

收录：SCI-EXPANDED(收录号：WOS:001533597200004)、、Scopus(收录号：2-s2.0-105008925651)、WOS

语种：英文

外文关键词：Worenine; Cisplatin; Non-small cell lung cancer; Mitophagy; HIF-1 alpha

外文摘要：Mitophagy has been implicated in chemoresistance in various cancers. Worenine, an active compound derived from Coptidis rhizome, has exhibited anti-tumor activity. We aimed to clarify the impacts of worenine on regulating the cisplatin (DDP) sensitivity and mitophagy in DDP-resistant non-small cell lung cancer (NSCLC) cells. The DDP-resistant NSCLC cell line (A549/DDP) were established via exposure to escalating DDP concentrations. Its resistance characteristics were confirmed by CCK-8 and flow cytometry assays. Moreover, the effects of worenine and/or DDP on the A549/DDP cells viability, colony formation abilities, apoptosis, and mitophagy-related proteins were evaluated. The A549/DDP cells were successfully established, with markedly higher IC50 value than A549 cells (25.67 mu M vs. 9.555 mu M). We noted that the HIF-1 alpha/BNIP3-mediated mitophagy was clearly activated in A549/DDP cells. Worenine decreased A549/DDP cell viability and colony formation abilities, promoted apoptosis, and suppressed HIF-1 alpha/BNIP3-mediated mitophagy. Importantly, worenine enhanced the responsiveness of A549/DDP cells to DDP. Besides, the suppressive impacts of worenine on A549/DDP cells were reversed by HIF-1 alpha overexpression. Our findings indicate that worenine may enhance the sensitivity of A549/DDP cells to DDP via suppressing HIF-1 alpha/BNIP3mediated mitophagy. Targeting mitophagy may be a promising therapeutic strategy for addressing chemoresistance in NSCLC.