文献类型：期刊文献

中文题名：灯盏花素对青春前期大鼠睾丸扭转复位双侧睾丸的保护作用

英文题名：Breviscapine protects bilateral testes of prepubertal rats after testicular torsion/detorsion

作者：吕晓晓[1];姚美玲[1];胡晓康[1];高力[1];张金萍[1]

机构：[1]绍兴文理学院医学院临床医学系

年份：2016

卷号：22

期号：4

起止页码：300

中文期刊名：中华男科学杂志

外文期刊名：National Journal of Andrology

收录：CSTPCD、、北大核心2014、CSCD2015_2016、Scopus、北大核心、CSCD、PubMed

基金：2015年浙江省大学生科技创新活动计划暨新苗人才计划(2015R428023)~~

语种：中文

中文关键词：灯盏花素;青春前期大鼠;睾丸扭转复位;缺血再灌注损伤

外文关键词：breviscapine; prepubertal rat; testicular torsion/detorsion; ischemia-reperfusion injury

中文摘要：目的:探讨灯盏花素青春前期大鼠睾丸扭转复位双侧睾丸的远期保护作用。方法:对建立左侧睾丸扭转复位动物模型,将32只4周龄健康SD雄性大鼠随机分为4组:假手术组、生理盐水组(腹腔内注射生理盐水2 mg/kg)、灯盏花素单次给药组(睾丸复位前30 min,腹腔缓慢注入灯盏花素2 mg/kg,给药体积为0.5 ml/100 g体重)和灯盏花素连续给药组(在单次给药组的基础上,于术后每天注射同等剂量灯盏花素注射液1次,连续7 d),每组8只。术后6周处死大鼠,取双侧睾丸和附睾,检测睾丸组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性与丙二醛(MDA)含量,测定精子浓度、存活率和活动率,行睾丸组织病理学观察。结果:与同侧生理盐水组比较,两给药组双侧SOD、T-AOC、NOS活性、精子浓度、精子存活率和精子活动率均升高,MDA含量下降,其中各检测指标除单次给药组健侧T-AOC活性、精子浓度、精子活动率、双侧NOS活性及连续给药组健侧NOS活性外差异均显著(P<0.05);与同侧单次给药组比较,连续给药组双侧SOD、T-AOC、NOS活性、精子浓度、精子存活率和精子活动率均升高,MDA含量下降,其中各项指标除健侧NOS活性外差异均显著(P<0.05)。生理盐水组可见双侧生精小管退变,间质水肿,且患侧受损明显大于健侧;两给药组使双侧睾丸扭转复位后损伤的组织学改变明显改善。结论:灯盏花素可通过有效清除氧自由基,减轻脂质过氧化损伤,对青春前期大鼠睾丸扭转复位双侧睾丸有明显的保护作用,且连续给药明显优于单次给药。

外文摘要：Objective： To investigate the long-term protective effect of breviscapine on the bilateral testes of prepubertal rats after testicular torsion / detorsion. Methods： Thirty-two four-week-old healthy male SD rats were equally randomized into groups A（sham operation）,B（normal saline）,C（single-dose breviscapine,injected intraperitoneally at 2 mg per kg of the body weight at 30 min before testicular detorsion）,and D（ continuous-dosing breviscapine,injected intraperitoneally at 2 mg per kg of the body weight at 30 min before testicular detorsion qd for 7 days after surgery）. At 6 weeks after establishment of the model of left testicular torsion /detorsion,the rats were sacrificed and the bilateral testes and epididymides harvested for detection of the total antioxidant capacity（ TAOC）,superoxide dismutase（ SOD） level,nitric oxide synthase（ NOS） activity,and malondialdehyde（ MDA） content in the testis tissue,determination of sperm concentration,viability and motility,and observation of the pathological changes of the testis tissue.Results： Compared with group B,both groups C and D showed obvious increases in the levels of SOD,T-AOC and NOS and the concentration,survival rate and motility of sperm,but a remarkable decrease in the MDA content,with statistically significant differences（P〈0. 05） except in T-AOC,sperm concentration and motility in the contralateral testis of group C and in NOS activity in the bilateral testes of group D. In comparison with the ipsilateral testis of group C,group D exhibited markedly elevated levels of SOD,T-AOC and NOS,increased sperm concentration,viability and motility,and reduced content of MDA in the bilateral testes,with statistically significant differences（ P〈0. 05） except in NOS activity in the contralateral testis. Bilateral seminiferous tubule degeneration and interstitial edema were observed in group B,more severe in the injured than in the contralateral testis. Obvious improvement was seen in the post-torsion / detorsion histological changes of the bilateral testis tissue in groups C and D. Conclusion： Breviscapine can obviously protect the bilateral testes of prepubertal rats after testicular torsion / detorsion by removal of oxygen free radicals and reduction of lipid peroxidation injury,and continuous dosing is even more effective than single-dose medication.