文献类型：期刊文献

英文题名：Postoperative Disseminated Mycoplasma hominis Infection with False-Negative Blood Cultures: A Case Report

作者：Yu, Yefu[1];Wang, Yanan[1];Mao, Weifang[1];Wang, Huiyu[1];Jin, Faxiang[1];Xu, Wenfang[1]

机构：[1]Shaoxing Univ, Affiliated Hosp, Dept Clin Lab, 999 Zhongxing South Rd, Shaoxing 312000, Peoples R China

年份：2025

卷号：18

起止页码：4549

外文期刊名：INFECTION AND DRUG RESISTANCE

收录：SCI-EXPANDED(收录号：WOS:001561606200001)、、Scopus(收录号：2-s2.0-105014797853)、WOS

基金：This study was supported by the Shaoxing Municipal Health and Family Planning Science and Technology Project (Grant No. 2024SKY084) and the Medical Science and Health Science Program of Zhejiang Province (Grant No. 2024KY485) .

语种：英文

外文关键词：Mycoplasma hominis; postoperative infection; hematogenous dissemination; MALDI-TOF MS

外文摘要：Background: Mycoplasma hominis commonly colonizes the genitourinary tract and primarily affects immunocompromised individuals. It is mostly confined to localized infections, with bloodstream dissemination being rare. Because of its fastidious nutritional requirements, the organism is seldom recovered by routine blood culture, and the absence of a cell wall renders it intrinsically resistant to many first-line antimicrobials. Consequently, the diagnosis and treatment of M. hominis bloodstream infections remain challenging. Case Description: A 72-year-old man developed persistent fever and marked systemic inflammation after lumbar spine surgery. Despite empirical broad-spectrum antibiotics, he progressed to severe incisional infection, pulmonary infection, and effusions in multiple serous cavities-including the left interlobar fissure, pleural space, and pericardium. Routine blood and urine cultures remained negative until two weeks after surgery, when M. hominis was first isolated from incisional exudate and definitively identified by MALDI-TOF MS. The patient ultimately recovered after surgical debridement and combination therapy with doxycycline plus moxifloxacin. During this period, we used Mycoplasma-specific liquid media combined with Columbia blood agar and subsequently recovered M. hominis from the patient's sputum, urethral swabs, and initially culture-negative blood samples. MALDI-TOF MS cluster analysis confirmed that all isolates belonged to a single clonal group responsible for disseminated infection. Conclusion: Immunocompromised patients with postoperative indwelling catheters constitute a high-risk population for hematogenous dissemination of M. hominis. In patients with persistent fever and negative routine cultures, M. hominis infection should be actively suspected. Timely targeted mycoplasma culture and MALDI-TOF MS confirmation are essential.