文献类型：期刊文献

英文题名：Constructing Linear-Oriented Pre-Vascularized Human Spinal Cord Tissues for Spinal Cord Injury Repair

作者：Fan, Caixia[1];Cai, Hui[2];Zhang, Lulu[2];Wu, Xianming[3];Yan, Junyan[1];Jin, Lifang[1];Hu, Baowei[1];He, Jiaxiong[1];Chen, Yanyan[2];Zhao, Yannan[3];Dai, Jianwu[2,3]

机构：[1]Shaoxing Univ, Sch Life & Environm Sci, Shaoxing 312000, Peoples R China;[2]Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Div Nanobiomed, Suzhou 215123, Peoples R China;[3]Chinese Acad Sci, State Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100080, Peoples R China

年份：2024

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：SCI-EXPANDED(收录号：WOS:001196317800001)、、EI(收录号：20241515872801)、Scopus(收录号：2-s2.0-85189547646)、WOS

基金：C.F. and H.C. contributed equally to this work. This work was supported by the National Key R&D Program of China (2023YFC2412503, 2023YFC2412504), National Science Foundation of China (NSFC) (grant no. 81901266, 81891002, 32330059), and CAS project for Young Scientists in Basic Research (Grant No. YSBR073). We also thanked the support from Shaoxing University (grant no. 20235005), Youth Innovation Promotion Association CAS (Grant No. Y202031), and CAMS Innovation Fund for Medical Sciences (2022-I2M-3-002).

语种：英文

外文关键词：neural regeneration; spinal cord injury; tissue engineering; vascularization

外文摘要：Repairing spinal cord injury (SCI) is a global medical challenge lacking effective clinical treatment. Developing human-engineered spinal cord tissues that can replenish lost cells and restore a regenerative microenvironment offers promising potential for SCI therapy. However, creating vascularized human spinal cord-like tissues (VSCT) that mimic the diverse cell types and longitudinal parallel structural features of spinal cord tissues remains a significant hurdle. In the present study, VSCTs are engineered using embryonic human spinal cord-derived neural and endothelial cells on linear-ordered collagen scaffolds (LOCS). Studies have shown that astrocytes and endothelial cells align along the scaffolds in VSCT, supporting axon extension from various human neurons myelinated by oligodendrocytes. After transplantation into SCI rats, VSCT survives at the injury sites and promotes endogenous neural regeneration and vascularization, ultimately reducing scarring and enhancing behavioral functional recovery. It suggests that pre-vascularization of engineered spinal cord tissues is beneficial for SCI treatment and highlights the important role of exogenous endothelial cells in tissue engineering. Pre-vascularized human spinal cord-like tissues (VSCT) that mimic the highly diverse cell types and longitudinal parallel structural features of natural spinal cord tissues are manufactured in vitro with late embryonic human spinal cord-derived cells (progenitors, neurons, astrocytes, and myelinating oligodendrocytes) and endothelial cells on linear-ordered collagen scaffolds (LOCS). image