文献类型：期刊文献

英文题名：Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

作者：Ji, Shaohui[1,2,4,5];Jin, Lifang[1,2,3];Guo, Xiangyu[1,2,4,5];Ji, Weizhi[1,2,4,6]

机构：[1]Chinese Acad Sci, Kunming Primate Res Ctr, Kunming 650223, Yunnan, Peoples R China;[2]Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China;[3]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[4]Yunnan Key Lab Anim Reprod Biol, Kunming, Zhejiang, Peoples R China;[5]Chinese Acad Sci, Grad Sch, Beijing, Peoples R China;[6]Kunming Biomed Int, Kunming, Cleveland, Peoples R China

年份：2010

卷号：46

期号：8

起止页码：693

外文期刊名：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

收录：SCI-EXPANDED(收录号：WOS:000281396600006)、、Scopus(收录号：2-s2.0-77957315549)、WOS

基金：We wish to acknowledge Mr. Li Jian for assistance in confocal microscopic analysis. This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425.

语种：英文

外文关键词：Hepatic progenitor cells; Bipotency; Serum free; Extracelullar matrix; Rhesus monkey

外文摘要：Studies with hepatic progenitor cells from non-human primates would allow better understanding of their human counterparts. In this study, rhesus monkey liver epithelial progenitor cells (mLEPCs) were derived from a small piece of newborn livers in chemical defined serum-free medium. Digested hepatic cells were treated in Ca(2+)-containing medium to form cell aggregates. Two types of cell aggregates were generated: elongated spindle cells and polygonal epithelial cells. Elongated spindle cells were expressed as vimentin and brachyury, and they were disappeared within 5 d in our cultures. The remaining type consisted of small polygonal epithelial cells that expressed cytokeratin 7 (CK7), CK8, CK18, nestin, CD49f, and E-cad, the markers of hepatic stem cells, but were negative for alpha-fetoprotein, albumin, and CK19. They can proliferate and be passaged, if on laminin or rat tail collagen gel, to initiate colonies. When cultured with dexamethasone and oncostatin M, the expression of mature hepatocyte markers, such as alpha-1-antitrypsin, intracytoplasmic glycogen storage, indocyanine green uptake, and lipid droplet generation, were induced in differentiated cells. If transferred onto mouse embryonic fibroblasts feeders, they gave rise to CK19-positive cholangiocytes with formation of doughnut-like structure. Thus, mLEPCs with bipotency were derived from newborn monkey liver and may serve as a preclinical model for assessment of cell therapy in humans.