文献类型：期刊文献

英文题名：LPS-induced TNF alpha factor (LITAF) in the snail Cipangopaludina chinensis: Gene cloning and its apoptotic effect on NCI-H446 cells

作者：Yang, Shoubao[1,2];Li, Peifen[1];Mi, Zhongxiang[1]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China;[2]Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China

年份：2012

卷号：32

期号：2

起止页码：268

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000300751800004)、、WOS

基金：This work was supported by the China Postdoctoral Science Foundation funded project (20100481431)and the Key Scientific Program of Shaoxing City, Zhejiang Province (2010A22019).

语种：英文

外文关键词：Cipangopaludina chinensis; LPS-induced TNF alpha factor (LITAF); CcLITAF; Apoptosis

外文摘要：LPS-induced TNF alpha factor (LITAF) is a transcription factor mediating TNF-alpha expression under LPS stimulation, and playing important roles in immune responses. In the present study, partial cDNA sequence of a LITAF (designated CcLITAF) gene was cloned and identified from snail Cipangopaludina chinensis. It contains an open reading frame of 348 nucleotides encoding a predicted protein of 115 amino acids, with a conserved LITAF domain at C-terminal, and shares a similarity ranging from 34% to 96% with other LITAF from oyster to mammals. CcLITAF mRNA ubiquitously expressed in all analyzed tissues. Interestingly, cLITAF could induce apoptosis in human tumor cell line, NCI-H446 cells, and caspase 3 play key roles in CcLITAF-mediated apoptosis. Present studies provide new insight into the biological function of CcLITAF. (C) 2011 Elsevier Ltd. All rights reserved.