文献类型：期刊文献

中文题名：中国东部沿海中小型河流鱼类的整体遗传多样性评价——以曹娥江为例

英文题名：EVALUATION OF GENERAL GENETIC DIVERSITY OF FISHES FROM MIDDLE AND SMALL RIVERS ON THE EAST CHINA——TAKING CAO’E RIVER AS AN EXAMPLE

作者：任岗[1];宣鑫玲[1];谢亚婷[1];李碧莹[1];陈旻[2];蔡亚军[2];沈文英[1]

机构：[1]绍兴文理学院生命科学学院,绍兴312000;[2]绍兴市水政渔业执法局,绍兴312000

年份：2020

卷号：44

期号：1

起止页码：67

中文期刊名：水生生物学报

外文期刊名：Acta Hydrobiologica Sinica

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：绍兴市科技计划项目(2018C20011)资助~~

语种：中文

中文关键词：曹娥江;遗传多样性;整体评价;人为干扰;细胞色素b基因

外文关键词：Cao’e River;Genetic diversity;General evaluation;Artificial interference factors;Mitochondrial cytochrome b

中文摘要：研究将曹娥江作为东部沿海中小型河流代表,基于线粒体细胞色素b基因(Cyt b)分子标记对其鱼类的整体遗传多样性水平进行了评估。结果表明,21个种26个群体鱼类Cyt b基因的单倍型多样性指数(h)和核苷酸多样性指数(π)水平分别为0.074—0.987和0.00019—0.00520,物种间遗传多样性水平差异较大;比较不同江段鱼类遗传多样性水平发现,曹娥江鱼类的单倍型多样性指数水平从河口到中下游再到上游逐渐降低(P<0.05)。比较同一物种的遗传多样性水平发现,曹娥江鱼类的核苷酸多样性指数水平低于长江、珠江等大型河流鱼类的相应指数水平(P<0.05),提示曹娥江鱼类的整体遗传多样性处于中等偏下水平,鱼类资源亟需科学的保护和修复。进一步分析人为扰动因素对曹娥江鱼类遗传多样性水平的影响发现,敏感型鱼类的单倍型多样性指数水平显著低于中等耐受型鱼类(P<0.05);麦穗鱼(Pseudorasbora parva)、光泽黄颡鱼(Pelteobagrus nitidus)和刺鳅(Mastacembelus aculeatus)3种鱼类的上游镜岭群体单倍型多样性指数和核苷酸多样性指数水平均低于其中游仙岩群体的相应指数水平。结果提示,水体污染、过度捕捞可能是造成目前曹娥江鱼类遗传多样性水平较低的主要原因。研究结果将为曹娥江乃至东部中小河流的鱼类资源的管理、保护和开发利用提供重要的理论依据。

外文摘要：The genetic diversity of fish is seriously inferred from human disturbance factors such as water pollution and habitat destruction.Recent studies on the genetic diversity of fishes in small and medium-sized rivers in eastern China have focused on simple species,but there has been less comprehensive evaluation of fish genetic diversity and its causes.In this study,Cao’e River was selected as the representation of middle and small rivers in East China to evaluate the general genetic diversities of its fishes using mitochondrial cytochrome b gene(Cyt b).The results showed that the haplotype diversity indices of Cyt b from the 21 species and 26 population were ranged from 0.074 to 0.987,and their nucleotide diversity indices were ranged from 0.00019 to 0.00520.The genetic diversities among different species were large.Comparing the genetic diversities of fishes in different sections of Cao’s River,the haplotype diversity indices of fishes decreased gradually from the estuary to upstream(P<0.05).The haplotype diversity indices in species populations from Cao’e River were significantly lower than that of same species from large rivers such as the Yangtze River and Yellow River(P<0.05).The haplotype diversity indices of sensitive fishes were significantly lower than those of middle tolerance fishes(P<0.05).Both the haplotype diversity indices and nucleotide diversity indices of three species,Pseudorasbora parva,Pelteobagrus nitidus and Mastacembelus aculeatus,in the populations from the upstream sampling site of Jinling were lower than those in the populations of same species from the sampling site of middle and lower reaches,Xianyan.This result implied that the overall genetic diversities of fishes from Cao’e River lied in middle or even low level,and that water pollution and overfishing might be the mainly reasons to reduce the genetic level of Cao’e River.In summary,our results provided an important theoretical basis for the management,protection,exploitation and utilization of fish resource of Cao’e River,and even of the middle and small rivers in East China.